黄皮叶甲醇提取物对油菜光活化化感活性的研究

用黄皮叶的甲醇提取物处理油菜种子,结果表明,光照与非光照条件下对油菜的生长均有一定的抑制作用。甲醇提取物浓度为4 mg/mL时,经紫外灯(波长365 nm,功率为60 W)照射3 h后培养4天,对油菜苗的根长、茎长和鲜重的抑制率分别为99.17%、99.20%、72.42%,高于非光照组的97.18%、82.07%、69.35%。毒力测定结果表明,粗提物对根长抑制率的IC50值光照组为0.44 mg/mL、非光照组为0.48 mg/mL;对茎长抑制率的IC50值光照组为0.99 mg/mL、非光照组为1.44 mg/mL,对幼苗鲜重抑制率的IC50值光照组为1.48 mg/mL、非光照组为1.51 mg/mL,且相关系数均达到0.90以上。并对黄皮叶甲醇提取物进行了初步分离,生测结果表明,乙酸乙酯层活性较好,浓度为2 mg/mL时对根长的抑制率可以达到100%。

Study on allelopathy activity of the methanol extract fromClausena lansium

The results showed that the methanol extract from Clausena lansium was very effective against the growth of the Brassica campestris under light and non-light.At the concentration of 4 mg/mL,the inhibition to root,shoot and wet weigh were 99.17%,99.20%,72.42% respectively after lighting 3 h,which was higher than the non-light97.18%,82.07%,69.35%.Results of toxicology showed that the IC_(50) were 0.44 mg/mL under light and 0.48 mg/mL under non-light respectively;the IC_(50) to shoot was 0.99 mg/mL under light and 1.44 mg/mL under non-light;the IC_(50) to wet weigh was 1.48 mg/mL under light and 1.51 mg/mL under non-light;and the correlation coefficient was all above 0.90.Preliminaryly seperated the extract and the results indicated that the extract of ethyl acetate was the best.