| 中华鳖致病性蜡样芽孢杆菌的分离鉴定与特性分析 |
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| 引用本文： | 谭爱萍,赵飞,姜兰,罗理,王伟利,彭华林,陈永乐,邹为民.中华鳖致病性蜡样芽孢杆菌的分离鉴定与特性分析[J].广东农业科学,2011,38(20). |
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| 作者姓名： | 谭爱萍 赵飞 姜兰 罗理 王伟利 彭华林 陈永乐 邹为民 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广东广州,510380
2. 广东省水生动物疫病预防控制中心,广东广州,510222 |
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| 基金项目： | 广东省重大科技兴海(兴渔)项目(070103); 广东省教育部产学研结合项目(2010A090200029) |
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| 摘 要： | 从患病中华鳖体内分离得到多株优势细菌,经感染性试验证明对健康中华鳖具有较强的致病性,且症状与自然发病中华鳖的症状相同。取代表菌株JY02、JY05、JY07和JY09进行形态学、溶血性、生理生化特性分析、16S rRNA基因序列分析和药物敏感性试验。研究结果表明:所分离的代表菌株JY02、JY05、JY07和JY09均为蜡样芽孢杆菌。药物敏感性试验结果显示,分离菌对庆大霉素、诺氟沙星、阿米卡星等8种药物敏感,对壮观霉素、万古霉素中度敏感,对复方新诺明、头孢曲松、氨曲南等6种药物产生耐药性。
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| 关 键 词： | 中华鳖 生理生化特性 16SrRNA基因 蜡样芽孢杆菌 |
Isolation and identification of Bacillus cereus from Trionyx sinensis |
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| TAN Ai-ping,ZHAO Fei,JIANG Lan,LUO Li,WANG Wei-Li,PENG Hua-lin,CHEN Yong-le,ZOU Wei-min.Isolation and identification of Bacillus cereus from Trionyx sinensis[J].Guangdong Agricultural Sciences,2011,38(20). |
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| Authors: | TAN Ai-ping ZHAO Fei JIANG Lan LUO Li WANG Wei-Li PENG Hua-lin CHEN Yong-le ZOU Wei-min |
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| Institution: | TAN Ai-ping1,ZHAO Fei1,JIANG Lan1,LUO Li1,WANG Wei-Li1,PENG Hua-lin2,CHEN Yong-le1,ZOU Wei-min1 (1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.Guangdong Provincial Aquatic Animal Epidemic Disease Prevention and Control Center,Guangzhou 510222,China) |
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| Abstract: | Some dominant bacterium were isolated from the focal area of the infected Trionyx sinensis.These dominant strains showed strong pathogenicity on healthy Trionyx sinensis in the artificial experiments.The symptoms of the artificial infected Trionyx sinensis and the natural infected ones were similar.We selected four typical strains named JY02,JY05,JY07,JY09.The strains were finally identified as Bacillus cereus that confirmed as the pathogen bacteria that caused shaking head disease through morphology observ... |
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| Keywords: | Trionyx sinensi physio-biochemical characteristic 16S rRNA gene Bacillus cereus |
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