改良CTAB法提取高质量香蕉叶片总RNA

以巴西香蕉叶片为试验材料,采用改良CTAB法提纯香蕉叶片总RNA.结果表明,改良CTAB法提取的香蕉叶片总RNA的28S、18SrDNA条带清晰、整齐,A260/A280值大于2.0,无需任何纯化处理即可用作香蕉MuACTIN基因的扩增模板,经RT-PCR扩增获得了带型清晰的目的条带,说明利用此法分离的RNA纯度和浓度符合RT-PCR的要求;而CTAB法提取的总RNA产量较低,且纯度不高.说明改良CTAB法能有效从富含多酚和多糖的香蕉叶片中提取高质量的总RNA.

Improved CTAB method for extracting high quality total RNA from banana leaves

In this study, improved CTAB method was applied to extract total RNA from banana Brazil leaves. The result showed that two bands of 28S rRNA and 18S rRNA extracted by improved CTAB method were appeared orderly, as well A260/A280value was more than 2.0. And using RNA as amplificating template of RT-PCR without any purification treatment, clear amplified band was acquired, which showed that purity and concentration of RNA extracted by improved CTAB method suited to apply to RT-PCR. But the total RNA extracted from CTAB method had lower yield and purity. Therefore, improved CTAB method could extract high quality total RNA of banana leaves.