|
|||||
|
|
| 新疆野生油菜与甘蓝型油菜属间杂交亲和性及杂种分子鉴定 | |
| 引用本文： | 郑卓,李健,李栒,官春云.新疆野生油菜与甘蓝型油菜属间杂交亲和性及杂种分子鉴定[J].湖南农业大学学报(自然科学版),2006,32(5):465-468. |
| 作者姓名： | 郑卓 李健 李栒 官春云 |
| 作者单位： | 1. 井冈山学院,生命科学学院,江西,吉安,343009;湖南农业大学,油料研究所,湖南,长沙,410128 2. 湖南农业大学,生物科学技术学院,湖南,长沙,410128 3. 湖南农业大学,油料研究所,湖南,长沙,410128 |
| 摘 要： | 采用蕾期剥蕾授粉的方法，研究了新疆野生油菜与甘蓝型油菜属间杂交亲和性．属间杂种F1植株形态表现一致，介于双亲之间，并且具有明显的杂种优势．从新疆野生油菜X湘油15号F1群体中选取9株进行RAPD分析，扩增出的带型完全一致，并且是父母本随机扩增DNA片段的综合，说明这9株全为真杂种．试验结果表明，蕾期授粉可以克服新疆野生油菜和甘蓝型油菜属间杂交的不亲和性，利用RAPD分子标记技术可以快速有效地鉴定远缘杂种． |
| 关 键 词： | 新疆野生油菜 甘蓝型油菜 远缘杂交不亲和 蕾期授粉 |
| 文章编号： | 1007-1032(2006)05-0465-04 |
| 修稿时间： | 2006年5月21日 |
Compatibility and Molecular Identification of Intergenetic Hybrids between the Xinjiang Wild Rape(Brassica arvensis L.) and Brassica napus L. |
|
| ZHENG Zhuo,LI Jian,LI Xun,GUAN Chun-yun.Compatibility and Molecular Identification of Intergenetic Hybrids between the Xinjiang Wild Rape(Brassica arvensis L.) and Brassica napus L.[J].Journal of Hunan Agricultural University,2006,32(5):465-468. | |
| Authors: | ZHENG Zhuo LI Jian LI Xun GUAN Chun-yun |
| Abstract: | The compatibility of Xinjiang wild rape(Brassica arvensis L.) and Brassica napus L.were studied by means of bud-pollination.All the intergenetic hybrids were morphologically intermediate between the female and the male.At the same time,the intergenetic hybrids show remarkable vegetative heterosis.RAPD analysis demonstrated that 9 F1 plants selected from intergenetic hybrids between Xinjiang wild rape and Xiangyou 15(B.napus L.) at random shared characteristic RAPD fragments of both parents,which indicated the 9 F1 plants were intergenetic hybrids.The results suggested that bud pollination could overcome the incompatibility between Xinjiang wild rape and B.napus L.,the RAPD was a simple and effective way to identify the intergenetic hybrids. |
| Keywords: | RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |