猪血液和肌肉组织DNA甲基化含量的测定

 摘要: 【目的】通过测定纯种亲本大白猪、长白猪、梅山猪及其正反交大白猪×长白猪、长白猪×大白猪、大白猪×梅山猪和梅山猪×大白猪等杂种猪血液和肌肉组织DNA甲基化含量，分别比较基于不同杂交组合和不同组织，亲本与子代之间在DNA甲基化含量上的差异,为揭示杂种优势的分子机制提供依据。【方法】采用高效液相色谱法测定血液和组织中甲基化含量。【结果】经过测定及计算，163份肌肉组织样平均甲基化含量为16.92%；182份血液样平均甲基化含量为6.49%，两者之间差异达到了极显著水平（p<0.01）。在相同组织不同杂交组合中杂种后代表现不同。同样地，在相同杂交组合不同组织之间杂种后代表现也不尽相同。【结论】杂种甲基化含量升高，可能是利用甲基化关闭了一些影响生长的不利基因的表达。杂种甲基化含量的变化表现为杂交组合和组织特异性。

Determination of DNA Methylation Contents Both in the Blood and Muscle Tissue of Pigs and Significant Difference Analysis

Abstract:【Objective】In this experiment, parental lines Large White、Landrace、Meishan and their hybrids Large White × Landrace、Landrace × Large White、Large White×Meishan、Meishan×Large White were used as the source of DNA samples for the determination of DNA methylation content both in the blood and muscle tissue of pigs, at the same time, the significant difference about DNA methylation content between parental lines and their hybrids, base on different hybrid systems and different tissues, were analyzed.【Method】High performance liquild chromatography, HPLC.【Result】The average DNA methylation content in the 163 samples of muscle tissue is 16.92%, while the average DNA methylation content in the 182 samples of blood is 6.49%, the difference between which is especially prominent (p<0.01). In the same tissue but different hybrid system, hybrids appear different. Similarly, in the same hybrid system but different tissue, hybrids are different too. 【Conclusion】The increase of DNA methylation percentage in hybrids may result in more silence expression of genes which are disbenificial to development. The hybrids methylation percentages differ in hybrid combination and tissue specificity.