感染鸡贫血病毒雏鸡接种新城疫疫苗后免疫保护效应及其机理研究

 雏鸡 1日龄人工感染鸡贫血病毒 (CAV) ,于 8日龄接种 L asota疫苗 ,免疫后 2 8d进行新城疫强毒 (v NDV)攻击 ,以同龄未感染 CAV和用 L asota疫苗免疫并经 v NDV攻毒雏鸡为对照 ,检测其免疫保护效应 ,胸腺和脾脏 T细胞数量及 T细胞增殖反应 ,法氏囊和脾脏 Ig G+、Ig M+、Ig A+抗体生成细胞数量 ,血清免疫球蛋白 Ig G、Ig M、Ig A含量 ,血凝抑制抗体 (HI)滴度等。结果表明 ,CAV人工感染并用新城疫 (ND)疫苗免疫雏鸡经 v NDV攻击后的免疫保护率为 6 0 % ,对照组雏鸡 v NDV攻击后免疫保护率为 10 0 % ,胸腺和脾脏 T细胞数量和 T细胞增殖反应、法氏囊和脾脏Ig G+ 、Ig M+ 、Ig A+ 抗体生成细胞数量、血清 Ig G、Ig M、Ig A含量及 HI抗体滴度 ,均较对照组雏鸡显著降低。说明感染鸡贫血病毒雏鸡接种新城疫疫苗后的免疫保护效应及免疫功能明显降低。

Study on Immunoprotective Efficacy and Machanism Post Newcastle Disease Vaccination of Chicken Infected with Chicken Anemia Virus

Chickens were infected with CAV at 1 day old and 8 days later the infected and uninfected chickens were vaccinated with Lasota vaccine. At 28 days post immunization these chickens were challenged with virulant newcastle disease virusv(vNDV). The immunoprotective efficiency, the content of IgG,IgM,IgA and hemoagglutination inhibution (HI) titer in serum, the number and proliferative response of T cells in thymus and spleen, the number of IgG ,IgM ,IgA antibody producing cells in Bursa Fabrisius and spleen were detected. The results demonstrated that the immunoprotective efficacy post challenge with vNDV was 60% of infected and immunized challenged chickens and it was 100% of uninfected and immunized challenged controls. At the same time the content of IgG,IgM,IgA and HI titer in serum, the number and proliferative response of T cells in thymus and spleen, the number of IgG ,IgM ,IgA antibody producing cells in Bursa Fabricius and spleen of infected and vaccinated challenged chickens were significantly dropped in comparison with uninfected and vaccinated challenged controls.