鸡miR-133a靶向调控BIRC5基因的表达

【目的】研究miR-133a在鸡不同组织中的表达情况，对baculoviral IAP repeat containing 5（BIRC5）进行靶基因的试验验证，探讨鸡miR-133a对BIRC5的靶向调控作用。【方法】采用生物信息学方法对miR-133a靶基因进行预测，并用双荧光素酶报告系统及点突变试验对BIRC5进行靶基因验证，同时运用实时荧光定量PCR检测miR-133a在鸡不同组织中的表达量。【结果】在鸡3′UTR数据库的11 891个基因中预测到287个miR-133a的靶基因；miR-133a在鸡的组织表达谱中显示其在肌肉中的表达量较高，尤其是在骨骼肌中表达量最高；报告基因试验显示BIRC5是miR-133a的靶基因，点突变试验证实了miR-133a在BIRC5上结合的靶位点。【结论】miR-133a是与鸡骨骼肌发育高度相关的miRNA，且鸡BIRC5基因是miR-133a的靶基因。

miR-133a Targets BIRC5 to Regulate Its Gene Expression in Chicken

【Objective】This study was conducted to investigate the expression pattern of miR-133a in various chicken tissues, and find whether baculoviral IAP repeat containing 5 (BIRC5) is the target gene of miR-133a.【Method】The bioinformatics methods were used to predict the target genes of miR-133a. Real-time PCR was used to detect the expression patterns of miR-133a of various tissues in chicken. Dual-luciferase reporter assays and site mutation assays were used to verify whether BIRC5 is a target gene of miR-133a. 【Result】Two hundred and eighty-seven genes of 11891 genes from 3′UTR database were predicted as target genes of miR-133a. The expression patterns of miR-133a in various chicken tissues showed that miR-133a was highly expressed in muscle especially in skeletal muscle. Reporter assays showed that BIRC5 was the target gene of miR-133a and the site mutation assays validated the target site of miR-133a in BIRC5. 【Conclusion】 miR-133a is a miRNA related with skeletal muscle development of chicken, and BIRC5 is a bona fide target gene in chicken.