3个小麦重组自交系群体抗赤霉病QTLs的SSR分析

 对3个重组自交系群体(RILs)苏麦3号/Alondra F_7、望水白/Alondra F_8、894037/Alondra F_8进行SSR分析,用单个标记方差及回归分析方法,结合接种鉴定资料,寻找与抗赤霉病性有关的的SSR分子标记。结果显示,在这3个抗病亲本的3B染色体上都存在一个主效抗赤霉病QTL,分别能解释2.6％～6.7％(苏麦3号)、47.4％(894037)、8.9％～27.0％(望水白)的抗性表型变异。在其它一些染色体上也发现一些微效QTLs,如2D、4B、4D、7A、6B染色体上,其中存在于4B染色体上的一个QTL来源于感病品种Alondra。在望水白/Alondra F_8群体中检测到了1个存在于2D染色体的抗赤霉病QTL,来源于抗病品种望水白,而在894037/Alondra F_8群体上检测到的1个存在于2D染色体的抗赤霉病QTL,则来源于感病品种Alondra,对其真实性将进一步研究。

SSR Markers for Fusarium Head Blight Resistance QTLs in Three Wheat Populations

DNA markers linked to QTLs controlling FHB resistance have been identified and mapped, and may be used to speed the introgression of resistance QTLs into commercial cultivars. This study was conducted to identify and locate the SSR markers linked FHB resistance QTLs in three wheat recombinant inbred populations. These populations were evaluated for reaction to inoculation with Fusarium graminearum in greenhouse and in field conditions. The first population of 161 recombinant inbred lines was from the cross Sumai3 (resistant)/Alondra (susceptible) . Two SSR markers associated with resistant QTLs, which were located on chromosome 3B, were found. These markers accounted for 2.6% -6.7% phenotypic variation. The second population of 147 recombinant inbred lines was from the cross 894037 (resistant )/Alondra (susceptible) . Total of 59 SSR primers were screened on this population. Seven SSR markers linked to resistant QTLs were found using ANOVA and regression analysis. QTLs on the chromosome 3B accounted for 47.4% phenotypic variation. Minor QTLs were also located on 2D, 7A, 6B and 4B chromosomes, and the resistance QTLs on 2D and 4B chromosomes were from Alondra. The last population of 80 recombinant inbred lines was from the cross Wang-shuibai (resistant)/Alondra (susceptible) . Total of 120 SSR primers were screened on this population. Eight SSR markers linked to resistant QTLs were found using ANOVA and regression analysis. These markers were located on 3B, 4B, 2D, 4D and 6D(uncertain) chromosomes, respectively. The resistant QTLs on chromosomes 4B and 6D(uncertain) are from Alondra. But it is interested that the resistance QTL on chromosome 2D is from Wangshuibai instead of Alondra. Test is needed in the future. SSR markers linked to resistance QTLs on chromosome 3B were found in all three populations, and account for higher phenotypic variation. So these markers should be useful in marker-assisted selection.