不同BMPRIB基因型小尾寒羊同期发情处理后FSHR和LHR表达量的研究

 【目的】根据小尾寒羊BMPRIB突变位点进行基因分型。研究发情期小尾寒羊BMPRIB基因型（BB、AB和AA）与FSHR和LHR mRNA表达量的关系。【方法】利用半定量PCR技术。【结果】在发情期BB型右侧卵巢FSHR 的mRNA水平（1.14±0.11）显著高于AA（0.44±0.11）和AB（0.36±0.08）型（P<0.01），但左侧卵巢在基因型间无显著性差异；BB型右侧卵巢LHR的mRNA水平（0.42±0.02）也显著高于AA（0.23±0.02）和AB（0.25±0.04）型（P<0.01），左侧卵巢各基因型间无显著性差异。【结论】FSHR和LHR的mRNA的高表达量可能是引起小尾寒羊较高的产羔数的原因之一。

Study on FSHR and LHR mRNA Levels of Different BMPRIB Genotypes from Small Tail Han Sheep During the Oestrum

【Objective】The relation between different BMPRIB genotypes in Small Tail Han sheep and FSH receptor and LH receptor mRNA levels during the oestrum was studied 【Method】Semi-quantitative PCR. 【Result】The results showed that the FSH receptor mRNA levels from the right ovary of BB (1.14±0.11) ewes were higher than those of AA (0.44±0.11) and AB (0.36±0.08) ewes (P<0.01). LH receptor mRNA levels from the right ovary of BB (0.42±0.02) ewes were significantly higher than those of AA (0.23±0.02) and AB (0.25±0.04) ewes (P<0.01), but was no significant difference in the left ovary among the genotypes during the oestrum was observed. 【Conclusion】It is concluded that the higher mRNA levels of FSH and LH receptor may be one of the reasons of fecund Small Tail Han sheep.