CYP86MF反义基因转化获得青花菜雄性不育植株

 通过根癌农杆菌LBA4404（含质粒反义CYP86MF基因片段）介导转化青花菜（Brassica oleracea L.var. italica Plenck）下胚轴，经卡那霉素选择压下连续选择、扩繁和生根培养，获得了青花菜转基因植株。经PCR、Southern blot、Northern blot检测证明，CYP86MF基因已经整合至转基因植株染色体中。经花器官观察，转基因植株中有雄蕊发育不良、花粉不萌发的植株。转基因植株自交不能结实，用转基因植株花粉对正常植株进行人工授粉，不能正常结实，表明转基因植株花粉是不育的。用正常花粉对转基因不育植株进行人工授粉，转基因不育植株能正常结实，表明转基因不育植株的雌性器官发育正常，其不育性与CYP86MF基因在转基因植株中的表达有关。

Male-Sterile Plants Induced by Anti-gene CYP86MF in Brassica oleracea var. Italica

An anti-gene CYP86MF was introduced into hypocotyls of broccoli(Brassica oleracea L.var. italica Plenck) with Agrobacterium tumefaciens, and transgenic plants were obtained by kanamycin selection. It was proved that anti-CYP86MF was integrated with chromosome of transgenic plant, according to the results of PCR, Southern blot, Northern blot. Plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male-sterile plants could set seeds if artificially pollinated with normal pollen, so the pistil of male-sterile plant was normal, and its sterility was induced by anti-CYP86MF.