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| 中国芸芥栽培品种亲缘关系的RAPD分析 | |
| 引用本文： | 钟军,李栒,官春云,戴林健,熊兴华,刘春林,周小云.中国芸芥栽培品种亲缘关系的RAPD分析[J].中国农业科学,2004,37(10):1434-1438. |
| 作者姓名： | 钟军 李栒 官春云 戴林健 熊兴华 刘春林 周小云 |
| 作者单位： | 1. 湖南农业大学基因工程省重点实验室,长沙,410128;湖南农业大学油料作物研究所,长沙,410128 2. 湖南农业大学油料作物研究所,长沙,410128 3. 湖南农业大学基因工程省重点实验室,长沙,410128 |
| 基金项目： | 湖南省科技厅资助项目(97JKY1005) |
| 摘 要： | 用100个随机引物对18个芸芥品种的基因组DNA进行扩增,结果有10个随机引物得到了稳定一致的RAPD谱带,扩增出的谱带数在2～17条之间,共扩增出91条谱带;采用UPGMA法对扩增出的谱带进行遗传聚类分析,在遗传距离(D)=0.1333处,将供试芸芥品种分为2类8组,揭示了供试芸芥品种的亲缘关系。 |
| 关 键 词： | 芸芥 RAPD DNA指纹图谱 聚类分析 |
RAPD Relative Analysis on Chinese Cultivars Variety of the Rocketsalad (Eruca sativa Mill) |
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| ZHONG Jun,LI Xun,GUAN Chun-yun,DAI Lin-jian,XIONG Xing-hua,LIU Chun-lin,ZHOU Xiao-yun.RAPD Relative Analysis on Chinese Cultivars Variety of the Rocketsalad (Eruca sativa Mill)[J].Scientia Agricultura Sinica,2004,37(10):1434-1438. | |
| Authors: | ZHONG Jun LI Xun GUAN Chun-yun DAI Lin-jian XIONG Xing-hua LIU Chun-lin ZHOU Xiao-yun |
| Institution: | ZHONG Jun1,2,LI Xun2,GUAN Chun-yun1,2,DAI Lin-jian1,XIONG Xing-hua1,LIU Chun-lin1,ZHOU Xiao-yun1 |
| Abstract: | The total DNA 18 rocketsalad (E.sativa Mill) were amplified with 100 random primers. The results showed that 10 primers produced reproducible amplified products and the bands of the products ranged from 2-17.Total 91 loci were identified, of which, 82 loci were polymorphism,accounted for 90.11%. According to the polymorphism, 18 rocketsalad could be divided into 2 types and 8 groups. This outcome was corresponding to the their geographical distribution. |
| Keywords: | E sativa Mill RAPD DNA fingerprinting Cluster analysis |
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