| 甘蔗ShSUT4基因及其5'侧翼序列的克隆与序列分析 |
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| 引用本文： | 吴自明,江安庆,张积森,叶冰莹,陈由强.甘蔗ShSUT4基因及其5'侧翼序列的克隆与序列分析[J].亚热带农业研究,2012,8(2):126-130. |
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| 作者姓名： | 吴自明 江安庆 张积森 叶冰莹 陈由强 |
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| 作者单位： | 福建师范大学生命科学学院；农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350108 |
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| 基金项目： | 福建省自然科学基金资助项目，农业部现代农业产业技术体系建设专项资金 |
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| 摘 要： | 以甘蔗品种FN28为材料,首次克隆甘蔗ShSUT4基因。测序结果表明,该基因约为4.8 kb,包含5个外显子和4个内含子,并包括完整的开放阅读框。该基因5'侧翼序列长度约为1.8 kb。采用PLACE、PlantCARE在线启动子预测工具分析表明,该序列含有启动子的特定结构,如TATA-box、CAAT-box等,还含有一些顺式作用元件如光响应元件、MYB结合位点、ABRE响应元件等,表明甘蔗ShSUT4基因的表达可能受光照、MYB和ABRE等的调控。
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| 关 键 词： | 甘蔗 蔗糖转运蛋白 基因克隆 序列分析 |
Cloning and sequence analysis of sucrose transporter 4 DNA and 5′ flanking sequence from sugarcane |
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| WU Zi-ming,JIANG An-qing,ZHANG Ji-sen,YE Bing-ying,CHEN You-qiang.Cloning and sequence analysis of sucrose transporter 4 DNA and 5′ flanking sequence from sugarcane[J].Subtropical Agriculture Research,2012,8(2):126-130. |
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| Authors: | WU Zi-ming JIANG An-qing ZHANG Ji-sen YE Bing-ying CHEN You-qiang |
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| Institution: | (College of Science,Fujian Normal University;Key Lab of Fujian Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fuzhou,Fujian 350108,China) |
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| Abstract: | A DNA encoding ShSUT4 was isolated from the leaf of sugarcane.The sequence had 5 exons and 4 introns with a size of 4.8 kb and included a whole open reading frame.The 5′ flanking sequence of ShSUT4 was about 1.8 kb.Based on the analysis of PLACE and PlantCARE,TATA-box,CAAT-box and some cis-acting elements such as light-induced responsive elements,MYB binding site,ABRE responsive were predicted in promoter sequence.So it implies that the ShSUT4 gene expression involved light,MYB,ABRE. |
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| Keywords: | sugarcane sucrose transporter gene cloning sequence analysis |
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