鸡肌肉组织中四环素类和氟喹诺酮类药物多残留检测

建立了能同时提取净化鸡肌肉组织中多残留的前处理并使用高效液相色谱分别检测的方法。样品经McIlvaine缓冲液、McIlvaine缓冲液-甲醇混合液提取后,过Oasis HLB固相萃取柱,5%甲醇(φ)淋洗,甲醇洗脱,40℃氮气吹干,磷酸-三乙胺缓冲液/乙腈复溶。样品四环素类药物用紫外检测器检测,波长365 nm,氟喹诺酮类药物用荧光检测器检测:激发波长280 nm,发射波长为480 nm。结果显示:鸡肌肉组织中4种四环素类药物OTC、TC的检测限是5μg/kg,CTC、DOXY的检测限是16μg/kg,回收率范围60.6%～89.1%,变异系数范围1.9%～14.3%;4种氟喹诺酮类药物的检测限均为2μg/kg,回收率范围77.5%～86.3%,变异系数范围2.8%～7.4%。说明该方法灵敏度高、适应性强,适用于鸡肌肉组织中上述4种四环素类药物和4种氟喹诺酮类药物残留的一次性检出。

Determination of tetracyclines and fluoroquinolones multiresidue in chicken muscle by High-Liquid Chromatography

In this study,the multiresidue of oxytetracycline(OTC),tetracycline(TC),chlortetracycline(CTC),doxytetracycline(DOXY),ofloxacin(OFL),norfloxacin(NOR),ciprofloxacin(CIP) and enrofloxacin(ENR) were simultaneously extraction and purification from chicken muscle by HPLC.The homogenized samples were extracted with perchloric acid to remove proteins and the extracts were partitioned with McIlvaine buffer and McIlvaine-methanol buffer.Further cleanup was perfomed on Oasis HLB solid-phase column.The cartridge was w...