秋水仙素诱导紫珠多倍体育种研究

试验采用秋水仙素处理紫珠的种子和幼苗茎尖生长点以诱导四倍体紫珠植株,并对秋水仙素的诱导时间和浓度进行了探讨,对诱导对象采用流式细胞分析仪鉴定和形态观察等方法进行了倍性鉴定.结果表明,秋水仙素成功地诱导出了四倍体紫珠植株,其中以6 mg/mL的秋水仙素对幼苗茎尖生长点处理48 h的效果较好,其形态变异率最高可达40.83％.四倍体紫珠植株较二倍体紫珠植株的形态有一定差异,表现为叶片普遍变大,气孔器保卫细胞较二倍体明显增大,叶绿体数目明显增多.同时,花径和果径平均增大37.26％和33.11％,观赏效果更加突出.

Study on Colchicines Induced Polyploid Breeding of Callicarpa bodinieri

The tetraploid Callicarpa bodinieri Lèvl.was induced by treating the seeds and shoot tip meristem with colchicine.The effects of concentration and treatment time of colchicine were studied;and the ploidy level was identified by morphological trait,cytological and flow cytometry techniques.The results showed that treating the shoot tip meristems with 6 mg/mL colchicine for 48 h has the best efficient with the mutation rate up to 40.83%.The tetraploid plant differed from the diploid morphologically and cytologicallly;it was with larger leaves,larger stomata and more chloroplast in stomata guard cell.The ornamental value of C.bodinieri was greatly improved as the flower diameter was increased by 37.26%;and the fruit diameter was increased by 33.11%.