黄芩苷微生物转化菌的筛选鉴定及发酵条件优化

通过罗尔夫青霉(Penicillium rolfsii)发酵培养基和发酵条件优化,来提升黄芩苷转化率。采用单因素方法对氮源、发酵接种量、初始pH发酵时间、发酵温度等因素进行考查,在此基础上进行L_(16)(4~5)正交试验优化。得到的优化发酵条件是黄芩粉20 g/L,(NH_4)_2SO_4 0.6 g/L,KH_2PO_4 0.2 g/L,醋酸钠0.1 g/L,维生素C 0.1 g/L,MgSO_4 0.1 g/L,pH 5.0,料液比为1∶30,接种量10%,装液量80 mL/250 mL,发酵温度35℃,发酵时间6 d,转速180 r/min。在最优条件下,黄芩素含量达到1.83 mg/mL。

Screening and Identify of the Baicalin Microbial Transformation and Optimization of Fermentation Conditions for Baicalein by Microorganism

The Penicillium rolfsii fermentation medium and conditions were optimized to increase the baicalin yield. Firstly, us-ing single-factor test, the effects of these 5 parameters, i.e., nitrogen source, the inoculation amount, initial pH of medium, fermentation time, fermentation temperature on the baicalin yield were studied. Then the key parameters of fermentation condi-tions were statistically optimized through orthogonal array experiments with 5 factors at 4 levels. The optimal conditions were Scutellaria baicalensis Georgi. power 20 g/L,(NH4)2SO40.6 g/L, KH2PO40.2 g/L, sodium salt 0.1 g/L, MgSO40.1 g/L, vitamin C 0.1 g/L, initial pH 5.0, solid liquid ratio 1:30, rotation speed 180 r/min, 10% inoculum size in 80 mL working volume(in 250 mL flasks) at 35℃ for 6 d. Using these conditions, the baicalein yield was 1.83 mg/mL.