金叶紫穗槐组织培养研究

以金叶紫穗槐(Amorpha fruticosa‘Jinye’)为外植体,探讨了不同生长调节对外植体分化、增殖和生根的影响。结果表明,(1)金叶紫穗槐组织培养最适宜的外植体是半木质化带芽茎段,以70%乙醇30 s+0.1%升汞(HgCl_2)消毒4~5 min为最好,外植体发芽率可达66.7%~70.0%。(2)适宜的增殖培养基为MS+6-BA 0.2 mg/L+NAA 0.01 mg/L,适宜的生根培养基为1/2MS+IBA 0.5 mg/L。(3)生根试管苗适宜的开瓶炼苗时间为2~3 d,在一年中的3~5月,选择基径≥0.3 mm的试管苗移栽到蛭石培养基上能够获得93.3%的成活率。

Tissue Culture of the Amorpha fruticosa 'Jinye'

Using Amorpha fruticosa 'Jinye' as explants, influences of different plant growth hormones on differentiation,multi-plication and rooting were examined. The results showed that①the optimal explant material was tender stem with single bud, sterilized 30 s in 70% ethanol and 4~5 min in 0.1% HgCl2,the germination rate of explants was 66.7%~70.0%. ②The optimal subculture was MS+6-BA 0.2 mg/L+NAA 0.01 mg/L,and rooting media was 1/2MS+IBA 0.5 mg/L.③the glass seedling rooting condign open the glass bottle hardened times was 2~3 d,than the diameter ≥0.3 mm glass seedling was transplanted on ver-miculite,the survive rates of glass seedling were 93.3% in march and may of a year.