| 新城疫病毒HN基因片段原核表达重组质粒的构建 |
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| 引用本文： | 赵永许,乔宪凤,刘西梅,华文君,周荆荣,郑新民,张淼涛.新城疫病毒HN基因片段原核表达重组质粒的构建[J].湖北农业科学,2008,47(5):506-508. |
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| 作者姓名： | 赵永许 乔宪凤 刘西梅 华文君 周荆荣 郑新民 张淼涛 |
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| 作者单位： | 1. 西北农林科技大学动物医学学院,陕西,杨凌,712100
2. 湖北省农业科学院畜牧兽医研究所/湖北省动物胚胎工程及分子育种重点实验室,武汉,430064 |
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| 摘 要： | 根据GenBank中发表的新城疫病毒F48E9基因序列设计引物,采用RT-PCR技术,成功获得了该病毒HN基因中两个不交叉的基因片段,长度分别为790bp和579bp.将这两段基因分别克隆到pGEM-T vector中,经酶切鉴定和测序后克隆到原核表达栽体pET-28a,PCR和酶切鉴定表明,克隆的两个新城疫病毒基因片段已经正确地插入到了原核表达载体pET-28a之中.
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| 关 键 词： | 新城疫病毒 HN基因片段克隆 原核表达 |
Construction of the Prokaryotic Expression Recombinant Plasmids Containing NDV Gene Fragments |
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| ZHAO Yong-xu,QIAO Xian-feng,LIU Xi-mei,HUA Wen-jun,ZHOU Jing-rong,ZHENG Xin-min,ZHANG Miao-tao.Construction of the Prokaryotic Expression Recombinant Plasmids Containing NDV Gene Fragments[J].Hubei Agricultural Sciences,2008,47(5):506-508. |
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| Authors: | ZHAO Yong-xu QIAO Xian-feng LIU Xi-mei HUA Wen-jun ZHOU Jing-rong ZHENG Xin-min ZHANG Miao-tao |
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| Institution: | ZHAO Yong-xu1,QIAO Xian-feng2,LIU Xi-mei2,HUA Wen-jun2,ZHOU Jing-rong2,ZHENG Xin-min2,ZHANG Miao-tao1(1.College of Veterinary Medicine,Northwest A&F University,Yangling 712100,Shanxi,China,2.Institute of Veterinary , Animal Science,Hubei Academy of Agricultural Science/Hubei Key Lab of Animal Embryo & Molecular Breeding,Wuhan 430064,China) |
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| Abstract: | Using Primers which were designed according to the sequence of HN gene of NDV F48E9 strain,two cDNA fragments 579 bp and 790 bp in length,of HN gene were successfully amplified by RT-PCR,and they are 579 bp and 790 bp in length.These two amplified cDNA fragments were cloned into pGEM-T vector.The results of identification by restriction endonuclease and sequencing analysis showed that they were successfully inserted into the expression vector pET-28a. |
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| Keywords: | NDV HN gene clone prokaryotic expression |
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