高效液相色谱法同时测定水稻及土壤中稻瘟灵和三环唑

采用高效液相色谱法,使用SunFireTMC18色谱柱和紫外检测器,样品用甲醇与丙酮的混合液提取,经弗罗里硅土净化后,以甲醇∶水( 80∶20,V/V)为流动相,流速为0.5 mL/min,在260 nm波长下对水稻及土壤中稻瘟灵与三环唑进行同时测定.结果表明,2种分析物得到了有效分离,稻瘟灵和三环唑的线性相关系数分别为0.999 8、0.9999,最低检出量分别为1.6、1.3 ng;水稻中稻瘟灵和三环唑平均回收率分别为84.78％～93.35％、86.75％～91.13％,相对标准偏差分别为2.15％～5.81％、2.46％～4.94％；土壤中稻瘟灵和三环唑平均回收率分别为89.65％～94.08％、88.81％～91.06％,相对标准偏差分别为2.19％～4.31％、3.08％～5.38％.该分析方法快速、简单、灵敏,具有良好的准确度和精密度,适用于常规分析检验.

Simultaneous Analysis of Isoprothiolane and Tricyclazole in Rice and Soil by High Performance Liquid Chromatography

The quantitative analysis method of isoprothiolane and tricyclazole was developed using high performance liquid chromatography(HPLC) with ultraviolet absorbance detector and a SunFireTMC18 column.Samples were extracted under ultrasonic with methanol and acetone,purified on the Florisil column.The mobile phase was methanol-water(80∶20,V/V) at a flow rate of 0.5 mL/min.The wavelength of detector was set at 260 nm.The results showed that these compounds could be effectively isolated.The linear correlation coefficient for isoprothiolane and tricyclazole was 0.999 8 and 0.999 9,respectively.The detection limit of isoprothiolane and tricyclazole was 1.6 and 1.3 ng,respectively.The recoveries of isoprothiolane and tricyclazole in rice were 84.78%～93.35% and 86.75%～91.13%,respectively,and relative standard deviations(RSDs) were 2.15%～5.81% and 2.46%～4.94%,respectively.The recoveries in soil were 89.65%～94.08% and 88.81%～91.06%,respectively;and RSDs were 2.19%～4.31% and 3.08%～5.38%,respectively.The method can be used for conventional analysis,which has rapid,simple,accurate and sensitive characteristics.