| 薄荷组织培养条件的选择 |
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| 引用本文： | 马同富,蔡健.薄荷组织培养条件的选择[J].湖北农业科学,2012,51(7):1471-1473,1480. |
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| 作者姓名： | 马同富 蔡健 |
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| 作者单位： | 1. 阜阳师范学院生命科学学院,安徽阜阳236037;抗衰老中草药安徽省工程技术研究中心,安徽阜阳236037
2. 阜阳师范学院生命科学学院,安徽阜阳,236037 |
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| 基金项目： | 安徽省教育厅重点科研项目，阜阳师范学院省级科研机构委托专项项目 |
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| 摘 要： | 考察了消毒方式、外植体部位、培养基配方等因素对薄荷(Mentha haplocalyx Brip.)组织培养的影响.结果表明,外植体先用体积分数70％的乙醇处理30 s后,再用1 mg/L HgCl2+2滴吐温80浸泡一定时间,消毒效果好于40 mg/L的漂白粉处理,适宜的HgCl2消毒时间分别为茎段、叶片、叶柄和已萌发的芽12 min;末萌发的芽10 min.茎段的不定芽诱导率和不定芽生长情况好于其他外植体.在薄荷旺盛生长期采集半木质化的茎段作为外植体进行诱导培养,不定芽萌芽早而且生长速度快.MS+0.50 mg/LBA+0.10 mg/L NAA是适合外植体不定芽诱导的培养基配方.
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| 关 键 词： | 薄荷(Mentha haplocalyx Brip.) 外植体 组织培养 |
The Optimization of Tissue Culture Conditions of Mint Explants |
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| MA Tong-fu
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CAI Jian.The Optimization of Tissue Culture Conditions of Mint Explants[J].Hubei Agricultural Sciences,2012,51(7):1471-1473,1480. |
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| Authors: | MA Tong-fu CAI Jian |
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| Institution: | 1(1.School of Life Sciences,Fuyang Teachers College,Fuyang 236037,Anhui,China; 2.Engineering Technology Research Center of Antiaging Chinese Herb,Fuyang 236037,Anhui,China) |
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| Abstract: | The effects of sterilizing methods,explant material,culture medium formula on tissue culture of mint(Mentha haplocalyx Brip.) was studied.The results showed that after explants being immersed in 70% ethanol,1 mg/L HgCl2+Tween 80 had better disinfection effect than 40 mg/L bleaching power.The treatment time of HgCl2 for steam,leaf,leafstalk and geminated bus should be 12 min;10 min for steam,especially steam collected in the vigorous growth period of mint as explants had higher inducing rate than other explants.MS+0.50 mg/L BA+0.10 mg/L NAA was the suitable medium for buds induction of mint explants. |
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| Keywords: | mint(Mentha haplocalyx Brip ) explant tissue culture |
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