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| 恩施碎米荠种子蛋白质双向电泳初探 | |
| 引用本文： | 雷红灵,付明,吴永尧.恩施碎米荠种子蛋白质双向电泳初探[J].湖北农业科学,2008,47(10). |
| 作者姓名： | 雷红灵 付明 吴永尧 |
| 作者单位： | 1. 湖南农业大学生物科学技术学院,长沙,410128;湖北民族学院湖北省生物资源保护与利用重点实验室,湖北,恩施,445000 2. 怀化学院生物工程系,湖南,怀化,418008 3. 湖北民族学院湖北省生物资源保护与利用重点实验室,湖北,恩施,445000 |
| 基金项目： | 国家民委科研项目，湖北省生物资源保护利用重点实验室开放课题 |
| 摘 要： | 对湖北恩施高硒地区生长的具有超富硒能力的植物恩施碎米荠的种子蛋白质用TCA-丙酮沉淀提取,等电聚焦采用固相梯度胶条,双向电泳分离蛋白质.结果表明,蛋白质样品制备的质量高;银染胶图背景干净,蛋白点清晰,且呈现较好的聚焦结果,无明显拖尾或横纹现象;蛋白质集中分布在pH5～10的范围之内,分子量主要在20～60 kμ的范围之内. |
| 关 键 词： | 恩施碎米荠 超富集硒植物 种子 蛋白质 双向电泳 |
Study on Two-dimension Electrophoresis of Seed Proteins of Cardamine enshiensis |
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| LEI Hong-ling,FU Ming,WU Yong-yao.Study on Two-dimension Electrophoresis of Seed Proteins of Cardamine enshiensis[J].Hubei Agricultural Sciences,2008,47(10). | |
| Authors: | LEI Hong-ling FU Ming WU Yong-yao |
| Institution: | LEI Hong-ling1,2,FU Ming3,WU Yong-yao2(1.College of Bioscience , Biotechnology,Hunan Agricultural University,Changsha 410128,China,2.Key Laboratory of Biological Resources Protection , Utilization of Hubei Province,Hubei University for Nationalities,Enshi 445000,Hubei,3.Department of Bioengineering,Huaihua University,Huaihua 418008,Hunan,China) |
| Abstract: | TCA-acetprecipitation was adopted to extract seed protein of Cardamine enshiensis,a Se hyperaccumulator,which was grown in the seleniferous area of Enshi,Hubei.Two-dimension Electrophoresis was adopted to separate seed protein and the first-dimensional electrophoresis was immobilized pH gradients isoelectric focusing.The result showed that the quality of sample of protein was high,silver staining of protein spots in the polyacrylamide gel were clear,and isoelectric focusing was effective,without tailing and... |
| Keywords: | Cardamine enshiensis Se hyperaccumulator seed protein two-dimension electrophoresis |
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