|
|||||
|
|
| 基本培养基及凝固剂对文心兰试管苗生长发育的影响 | |
| 引用本文： | 何松林,十鸟三和子,孔德政,张启翔,田中道男.基本培养基及凝固剂对文心兰试管苗生长发育的影响[J].北京林业大学学报,2001,23(1):29-31. |
| 作者姓名： | 何松林 十鸟三和子 孔德政 张启翔 田中道男 |
| 作者单位： | 1. 河南农业大学林学园艺学院，450002，郑州 2. 日本国香川县笠田高等学校，761-1503，香川 3. 北京林业大学园林学院，100083，北京 4. 日本国立香川大学农学部，761-0795，香川 |
| 基金项目： | 留学回国人员基金资助项目 |
| 摘 要： | 以薄叶系文心兰Alha‘lwanaga’茎尖诱导形成的原球茎 (Protocomlikebody ,以下简称PLB)进一步分化形成的幼苗为试材 ,比较不同培养基类型 (MS ,1/ 2MS ,VW )及不同种类凝固剂 (琼脂、Gellangum)对幼苗生长的影响 .结果表明 :实验用基本培养基类型中 ,幼苗的生长以MS较好 ,Gellangum作凝固剂时优于琼脂 .同时证明 ,培养时幼苗愈小 ,其基部PLB形成率愈高 .幼苗分化形成后 ,进一步培养以株高 3cm左右为宜 |
| 关 键 词： | 文心兰 组织培养 培养基 凝固剂 |
Effects of different media and coagulant on the growth of in vitro Oncidium shoots. |
|
| He Songlin, M. Jutori, Kong Dezheng, Zhang Qixiang, M. Tanaka. Journal of Beijing Forestry University.Effects of different media and coagulant on the growth of in vitro Oncidium shoots.[J].Journal of Beijing Forestry University,2001,23(1):29-31. | |
| Authors: | He Songlin M Jutori Kong Dezheng Zhang Qixiang M Tanaka Journal of Beijing Forestry University |
| Abstract: | The effects on the growth of in vitro Oncidium shoots were studied with different media (MS, 1/2MS and VW) and coagulant (Difco agar and Gellan Gum) by using different size of shoots (0.6, 2.0, 3.2,and 4.8cm) of Oncidium Alha‘lwanaga' induced from PLB. The results indicate that MS is the best medium for in vitro shoot growth, Gellan Gum is better than agar. The smaller the young seedlings are, the higher the forming rate of the base PLB is. The optimum seedling height is about 3?cm when the formation finished and the seedling will be cultivated. |
| Keywords: | Oncidium tissue culture medium coagulant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |