| 核桃子叶RNA提取方法的研究 |
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| 引用本文： | 董宁光,高英,裴东.核桃子叶RNA提取方法的研究[J].北京林业大学学报,2011,33(6):98-101. |
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| 作者姓名： | 董宁光 高英 裴东 |
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| 作者单位： | 1北京林业大学林学院2 中国林业科学研究院林业研究所，林木遗传育种国家重点实验室 |
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| 基金项目： | 国家自然科学基金项目(31171933) |
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| 摘 要： | 针对核桃子叶富含酚类、色素、多糖、油脂等物质的特点,以常用的Trizol法、异硫氰酸胍法、SDS法和改良的CTAB法4种方法提取核桃子叶总RNA,通过RNA产率、纯度、电泳图谱来确定适于核桃子叶RNA提取的方法。结果表明：Trizol法和SDS法所提取的RNA纯度较低;异硫氰酸胍法所提取的RNA降解严重,RNA以小分子...
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| 关 键 词： | 核桃 子叶 RNA提取 改良CTAB法 |
| 收稿时间： | 1900-01-01 |
Extracting methods of total RNA in cotyledon of walnut. |
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| DONG Ning-guang ,GAO Ying,PEI Dong.Extracting methods of total RNA in cotyledon of walnut.[J].Journal of Beijing Forestry University,2011,33(6):98-101. |
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| Authors: | DONG Ning-guang GAO Ying PEI Dong |
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| Institution: | 1 College of Forestry, Beijing Forestry University, 100083, P. R. China; 2 Research Institute of Forestry, State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing，100091, P. R. China. |
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| Abstract: | Total RNA was isolated from walnut cotyledon by the methods of Trizol,Guanidine Thioeyanate,SDS and improved CTAB,according to walnut cotyledon characteristics of rich phenols,pigment,polysacchride and oil.The method of RNA isolation suitable for cotyledon of walnut was established through the rate of yield,purity and electrophoresis.The results showed that the purity of RNA isolated by Trizol and SDS reagent was lower than improved CTAB.RNA isolated by the Guanidine Thioeyanate dispersed in the pattern of ... |
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| Keywords: | walnut cotyledon isolation of RNA improved CTAB methods |
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