‘小黄’菊遗传转化再生体系的建立

以‘小黄’菊茎段、叶盘为外植体 ,选用MS培养基为基本培养基 ,附加激素 6 BA和NAA ,通过研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响 ,建立了较好的遗传转化再生体系 .试验结果表明‘小黄’菊茎段、叶盘最适分化培养基分别为MS +6 BA 2mg L +NAA 1mg L和MS +6 BA 1mg L +NAA 0 1mg L ;小苗的最佳生根培养基为 1 2MS +NAA 0 1mg L ,生根率可达 10 0 % ;4 0mg L卡那霉素可以抑制茎段的分化 ,10mg L卡那霉素可以抑制叶片的分化 ,2 0mg L卡那霉素可以抑制分化小苗的生根 .

Establishment of a genetic transformation system in Dendrathema grandiflorum‘Xiao Huang'

Adventitious shoots were induced from both stem and leaf explants of Dendrathema grandiflorum ‘Xiao Huang' in this study. The optimized medium for stem and leaf explants contains Murashige & Skoog basal medium supplemented with 2 mg/L 6 BA + 1 mg/L NAA, and 1 mg/L 6 BA +0 1 mg/L NAA, respectively. More shoots are generated from leaf than from stem explants. The medium for rooting of shoots is optimized as 1/2 Murashige & Skoog basal medium supplemented with 0 1 mg/L NAA, with a rooting efficiency of 100%. Kanamycin at 40 mg/L and 10 mg/L in the medium gives effective inhibition to the regeneration of shoots from the stem and leaf explants, respectively. In addition, Kanamycin at 20 mg/L in the medium shows obvious inhibition to the regeneration of roots from the shoots.