| 西瓜RAPD-PCR体系的正交优化研究 |
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| 引用本文： | 张彦萍,刘海河.西瓜RAPD-PCR体系的正交优化研究[J].河北农业大学学报,2005,28(4):51-53. |
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| 作者姓名： | 张彦萍 刘海河 |
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| 作者单位： | 河北工程学院,河北,邯郸,057150 |
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| 摘 要： | 采用正交试验设计的方法,建立了西瓜RAPD分析的优化反应体系,即20μL反应体系中含有1×buffer,dNTP250μmol/L、Primer0.4μmol/L、MgCl22.0mmol/L、Taq酶1U和模板DNA20~40ng。适宜的扩增条件为94℃5min;94℃30s,37℃45s,72℃90s,45个循环;72℃5min;4℃保存。
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| 关 键 词： | 西瓜 RAPD 体系优化 |
| 文章编号： | 1000-1573(2005)04-0051-03 |
Optimization of RAPD-PCR system with orthogonal design in watermelon |
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| ZHANG Yan-ping,LIU Hai-he.Optimization of RAPD-PCR system with orthogonal design in watermelon[J].Journal of Agricultural University of Hebei,2005,28(4):51-53. |
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| Authors: | ZHANG Yan-ping LIU Hai-he |
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| Abstract: | The optimal RAPD-PCR system in watermelon was established with orthogonal design. In a total volume of 20 L RAPD-PCR system, it contains 1buffer,250 mol/L dNTP,0.4 mol/L Primer,2.0 mmol/L MgCl_2,1 U Taq DNA polymerase and 2040 ng template DNA. The suitable PCR procedure is one cycle denaturing at 94 for 5 min;45 cycles which involves denaturing at 94 for 30 s,annealing at 37 for 45 s and extending at 72 for 90 s, one cycle extending at 72 for 5 min, and then kept at 4. |
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| Keywords: | watermelon RAPD system optimization |
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