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| 利用CTAB法提取红掌不同部位基因组DNA | |
| 引用本文： | 范永山,刘海英,李卫国,王玉芹.利用CTAB法提取红掌不同部位基因组DNA[J].河北农业大学学报,2006,29(3):36-38. |
| 作者姓名： | 范永山 刘海英 李卫国 王玉芹 |
| 作者单位： | 1. 唐山师范学院,生物系,河北,唐山,063000 2. 唐山市农业科学研究院,河北,唐山,063000 3. 河北省定州中山路东街中学,河北,定州,071001 4. 河北科技报社,河北,石家庄,050000 |
| 基金项目： | 唐山师范学院校科研和教改项目 |
| 摘 要： | 采用CTAB法,对红掌的叶、叶柄、肉穗花序、佛焰苞、根等5个部位进行基因组DNA提取,利用紫外分光光度法、琼脂糖凝胶电泳法和RAPD扩增法检测DNA的质量,结果表明,CTAB法适合红掌根和叶的基因组DNA提取,没有蛋白质、酚及色素等杂质的污染,佛焰苞的DNA中有少量蛋白质污染,叶柄DNA中含有较多的蛋白质、酚等杂质,而从肉穗花序中没有提取到基因组DNA。 |
| 关 键 词： | 红掌(Anthurium and raeanum) DNA提取 CTAB法 RAPD |
| 文章编号： | 1000-1573（2006）03-0036-03 |
| 收稿时间： | 2005-10-17 |
| 修稿时间： | 2005-10-17 |
Isolation of genomic DNA from different organs in Anthurium andraeanum by CTAB method |
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| FAN Yong-shan,LIU Hai-ying,LI Wei-guo,WANG Yu-qin.Isolation of genomic DNA from different organs in Anthurium andraeanum by CTAB method[J].Journal of Agricultural University of Hebei,2006,29(3):36-38. | |
| Authors: | FAN Yong-shan LIU Hai-ying LI Wei-guo WANG Yu-qin |
| Institution: | 1. Biological Department, Tangshan Teachers College, Tangshan 063000, China; 2. Tangshan Agricultural Institute, Tangshan 063000, China; 3. DongJie Middle Scheol of Dingzhou, Dingzhou 071001, China; 4. Newspaper Institute of Science and Technology of Hebei, Shijiazhnang 050000, China |
| Abstract: | Genomic DNAs from lamina,leaf stalk,root,spathe,and spadix of Anthurium andraeanum were extracted by the CTAB method.The qualities of DNA were detected by ultraviolet spectrophotometry,agarose gel electrophoresis and RAPD amplification,and the results showed that the CTAB method was adaptive to the genomic DNA extraction of root and lamina because there was no contamination of protein,phenol etc.The genomic DNA from spathe had a few protein contaminations and the genomic DNA from the leaf stalk of A.andraeanum mixed with a lot of protein,phenol and other contaminations,but there was no genomic DNA isolated from spadix by the CTAB method. |
| Keywords: | Anthurium andraeanum genomic DNA extraction CTAB method RAPD |
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