| 中国小麦条锈菌4个流行小种的RAPD标记 |
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| 引用本文： | 曹丽华,康振生,赵 杰.中国小麦条锈菌4个流行小种的RAPD标记[J].西北农林科技大学学报(社会科学版),2004,32(7):37-40. |
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| 作者姓名： | 曹丽华 康振生 赵 杰 |
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| 作者单位： | 西北农林科技大学,植保学院,生物技术中心,陕西,杨凌,712100 |
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| 基金项目： | 国家"973"计划项目(G200016201) |
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| 摘 要： | 以210条随机引物,对目前中国小麦条锈菌(Pucciniastriiformisf.sp.Tritici)的主要优势菌系进行了RAPD片段的规模筛选,寻找到了条中31号、条中29号、条中23号、水源类型4个流行生理小种的特异性RAPD标记。此结果表明,通过寻找小麦条锈菌生理小种的特异性RAPD片段,能够建立起中国小麦条锈菌生理小种的分子检测体系。
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| 关 键 词： | 小麦条锈菌 RAPD 分子标记 |
| 文章编号： | 1671-9387(2004)07-0037-04 |
| 收稿时间： | 6/9/2003 12:00:00 AM |
| 修稿时间： | 2003年6月9日 |
RAPD markers of Puccinia striiformis f.sp.tritici in China |
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| CAO Li-hua,KANG Zheng-sheng,ZHAO Jie,HUANG Li-li,WEI Guo-rong.RAPD markers of Puccinia striiformis f.sp.tritici in China[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(7):37-40. |
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| Authors: | CAO Li-hua KANG Zheng-sheng ZHAO Jie HUANG Li-li WEI Guo-rong |
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| Institution: | (Biotech Center,College of Plant Protection,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | Rapid identification of the races of Puccinia striiformis f.sp.tritici by molecular technique was very important to monitor and control wheat stripe rust in China.In this paper,five physiological races of Puccinia striiformis f.sp.tritici were analysed with 210 primers by RAPD technique.The special RAPD fragments of CY31,CY29,CY23 and Shuiyuan pathotype were founded successfully.The results here indicates that a molecular identification system for the races of Puccinia striiformis f.sp.tritici in China could be established through cosmically searching special RAPD fragments of different races. |
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| Keywords: | wheat stripe rust RAPD molecular marker |
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