中国李原生质体培养及植株再生

对中国李（ＰｒｕｎｕｓｓａｌｉｃｉｎａＬｉｎｄｌ．）原生质体分离和培养的有关因素进行了研究。以悬浮培养物为材料，在适宜的酶解液中酶解１２ｈ，原生质体产量和活力分别达到３×１０７个／ｇ和９５％．以改良ＭＳ为基本培养基，在培养初期加２，４－Ｄ１．０ｍｇ／Ｌ，ＢＡ０．５ｍｇ／Ｌ，培养３０ｄ后将ＢＡ调至１．０ｍｇ／Ｌ，以０．５５ｍｏｌ／Ｌ葡萄糖调节渗透压，在２×１０５个／Ｌ的植板密度下液体浅层培养５０ｄ后形成了微愈伤组织。微愈伤组织转至固体培养基上继代培养，在分化培养基上分化出不定芽，在生根培养基上生根形成完整植株。

Protoplast Culture and Plant Regeneration of Chinese Plum

Some conditions of protoplast isolation and culture of Chinese plum (Prunus salicina Lindl.) were studied.The protoplast yield and viability of suspension cultures could amount to 3107 protoplasts/g and 95%,respectively,when incubated in suitable enzyme solution for 12 hours.Modified MS medium,with 1.0 mg/L 2,4D and 0.5 mg/L BA during beginning stage and BA concentration adjusted to 1.0 mg/L after 30 days culture,was a suitable culture medium.Osmoticum of the medium was adjusted to 0.55 mol/L with glucose.Microcalli were developed after 50 dayss culture in a liquid layer with plating density of 2105/L.The microcalli were subcultured on solid medium and developed adventitious buds on diffentiation medium.Eventually,shoots rooted and developed into whole plantlets on rooting medium.