| 摘要: |
| 对中国李（Prunus salicina Lindl.）原生质体分离和培养的有关因素进行了研究。以悬浮培养物为材料，在适宜的酶解液中酶解12h，原生质体产量和活力分别达到3×107个/g和95%.以改良MS为基本培养基，在培养初期加2,4-D1.0mg/L,BA0.5mg/L培养30d后将BA调至1.0mg/L,以0.55mol/L葡萄糖调节渗透压，在2×105个/L的植板密度下液体浅层培养50d后形成了微愈伤组织。微愈伤组织转至固体培养基上继代培养，在分化培养基上分化出不定芽，在生根培养基上生根形成完整植株。 |
| 关键词: 中国李，原生质体分离，原生质体培养，植株再生 |
| DOI： |
| 分类号:S6 |
| 基金项目: |
|
| Protoplast Culture and Plant Regeneration of Chinese Plum |
|
|
| Abstract: |
| Some conditions of protoplast isolation and culture of Chinese plum (Prunus salicina Lindl.) were studied.The protoplast yield and viability of suspension cultures could amount to 3×107 protoplasts/g and 95%,respectively,when incubated in suitable enzyme solution for 12 hours.Modified MS medium,with 1.0 mg/L 2,4-D and 0.5 mg/L BA during beginning stage and BA concentration adjusted to 1.0 mg/L after 30 days culture,was a suitable culture medium.Osmoticum of the medium was adjusted to 0.55 mol/L with glucose.Microcalli were developed after 50 dayss culture in a liquid layer with plating density of 2×105/L.The microcalli were subcultured on solid medium and developed adventitious buds on diffentiation medium.Eventually,shoots rooted and developed into whole plantlets on rooting medium. |
| Key words: Chinese plum (Prunus salicina),protoplast isolation,protoplast culture,plant regeneration |
