| TDZ对仙客来不同外植体再生的影响 |
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| 引用本文： | 周连霞,马锋旺,陈登文.TDZ对仙客来不同外植体再生的影响[J].西北农林科技大学学报(社会科学版),2006,34(4):39-42. |
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| 作者姓名： | 周连霞 马锋旺 陈登文 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨凌,712100
2. 杨凌职业技术学院,农学系,陕西,杨凌,712100 |
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| 基金项目： | 教育部留学回国人员科研启动基金;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 在无菌条件下培养仙客来种子,并以无菌萌发植株的子叶节、子叶、叶片及叶柄为外植体进行芽分化诱导。试验结果表明,不同浓度TDZ对各外植体的诱导效果不同,用子叶节、子叶、叶片及叶柄均可诱导出再生芽,但以叶片为材料的诱导效果最佳,其中1.0 m g/L TDZ对叶片再生芽的诱导效果最好,而子叶以0.5 m g/LTDZ、子叶节和叶柄以0.2 m g/L TDZ诱导再生芽的效果较好。
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| 关 键 词： | 仙客来 TDZ 外植体 植株再生 |
| 文章编号： | 1671-9387(2006)04-0039-04 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005年8月10日 |
Effect of TDZ on bud regeneration from different explants in Cyclamen persicum |
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| ZHOU Lian-xia,MA Feng-wang,CHEN Deng-wen,QIN Jing-yuan,CHENG Rui-hong.Effect of TDZ on bud regeneration from different explants in Cyclamen persicum[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(4):39-42. |
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| Authors: | ZHOU Lian-xia MA Feng-wang CHEN Deng-wen QIN Jing-yuan CHENG Rui-hong |
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| Institution: | 1(1 College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Department of Agronomy,Yangling Vocational and Technical College,Yangling,Shaanxi 712100,China) |
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| Abstract: | Bud regeneration of Cyclamen persicum was studied by using aseptic seedling's hypocotyls,cotyledon,leaf and petiole from the seeds.The results showed that thidiazuron(TDZ) concentration and explant type significantly affected bud regeneration.Bud formation was observed in media containing TDZ regardless of explant type and the highest percentage of bud formation and bud number was obtained when cultured on media containing 1.0 mg/L TDZ from leaf explant.The high percentage of bud regeneration was obtained from cotyledon explants cultured on media supplemented with 0.5 mg/L,and both hypocotyls and petiole with 0.2 mg/L TDZ. |
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| Keywords: | TDZ |
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