分子生物学新方法在根瘤菌分类中的应用

随着分子生物学技术的发展，一些全新的方法应用于根瘤菌分类，使根瘤菌分类的研究得到了迅速发展，从过去的传统分类过渡到了以遗传特征和系统发育为主要依据的现代系统分类。研究在参考大量文献的基础上，概述了全细胞可溶性蛋白电泳、多位点酶电泳、ＤＮＡＧ＋Ｃ摩尔百分含量测定及ＤＮＡ－ＤＮＡ杂交、限制性片段长度多态性分析、随机扩增ＤＮＡ片段的多态性、基因外回文重复序列ＰＣＲ扩增技术及１６ＳｒＤＮＡ序列分析确定根瘤菌系统发育等７种分子生物学方法的原理、特点，以及在根瘤菌分类研究中的应用现状和前景。

Applications of Molecular\n\rBiological Methods on Taxonomy of Rhizobia

With the development of molecular biological techniques,some new molecular biological methods are applied to the taxonomy of rhizobia.These methods advance to the rapid development of the research of taxonomy of rhizobia,from the traditional taxonomy in the past to the modern systematic taxonomy based on genetic characteristics and phylogenetic relationships.This paper reports on the basis of a large amount of literatures,introduces the basic concepts,principles,characteristics,and their application at the present and in the future on taxonomy of rhizobia of some new molecular biological methods such as whole cell protein electrophoresis,multilocus enzyme electrophoresis (MLEE),DNA G C mol%,DNA DNA hybridization,restriction fragment length polymorphism (RFLP),randomly amplified polymorphic DNAs (RAPD),repetitive extragenic palindrome PCR (REP PCR),16s rDNA sequencing.