| 酵母双杂交筛选与RSA14-44相互作用的蛋白 |
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| 引用本文： | 袁莉刚,田永强,刘英,缪时英,王琳芳.酵母双杂交筛选与RSA14-44相互作用的蛋白[J].甘肃农业大学学报,2004,39(6):679-682. |
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| 作者姓名： | 袁莉刚 田永强 刘英 缪时英 王琳芳 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070
2. 中国协和医科大学分子生物学国家重点实验室,北京,100005 |
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| 基金项目： | 国家重点基础研究发展规划项目(G1999055901) |
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| 摘 要： | 构建了包含RSA14-44cDNA的pAS2-1-14诱饵质粒,并进行了人睾丸cDNA文库的酵母双杂交筛选,获得了一条编码人20S蛋白酶体蛋白PSMB5的cDNA片段;用所获得的序列编码该蛋白3’端编码框内全部氨基酸(191个氨基酸);首次证明RSA14-44与PSMB5在酵母细胞中存在相互作用。
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| 关 键 词： | RSA-14-44 酵母双杂交 PSMB5 |
| 文章编号： | 1003-4315(2004)06-0679-04 |
Screening of the protein interacting with RSA-14-44 using yeast two hybrid method |
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| YUAN Li-gang,TIAN Yong-qiang,LIU Ying,MIAO Shi-ying,WANG Lin-fang.Screening of the protein interacting with RSA-14-44 using yeast two hybrid method[J].Journal of Gansu Agricultural University,2004,39(6):679-682. |
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| Authors: | YUAN Li-gang TIAN Yong-qiang LIU Ying MIAO Shi-ying WANG Lin-fang |
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| Institution: | YUAN Li-gang1,2,TIAN Yong-qiang2,LIU Ying1,MIAO Shi-ying2,WANG Lin-fang2 |
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| Abstract: | RSA14-44 is a new gene located on the fourth chromosome of rat and obtained from the rat testis tissue. To obtain the protein interacting with RSA14-44, the recombinant bait plasmid pAS2-1-14 was constructed and transformed into yeast strain Y190 to test pAS2-1-14 for no autonomous activation. One true positive, which obtained by screening the Human testis cDNA libraries, was confirmed to be PSMB5 by sequencing and protein BLAST. It concluded that RSA14-44 could interact with PSMB5. |
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| Keywords: | RSA14-44 yeast two hybrid method PSMB5 |
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