| 金丝桃素注射剂的制备及其含量的测定 |
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| 引用本文： | 谢等龙,梁剑平,胡永浩,尚若峰,华兰英,王学红.金丝桃素注射剂的制备及其含量的测定[J].甘肃农业大学学报,2008,43(2):31-33. |
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| 作者姓名： | 谢等龙 梁剑平 胡永浩 尚若峰 华兰英 王学红 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070
2. 中国农业科学院兰州畜牧与兽药研究所,甘肃,兰州,730050 |
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| 摘 要： | 采用高效液相色谱法(HPLC法)测定所制备的金丝桃注射液中金丝桃素的含量,测定时采用Chrom-sphere C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-乙腈-1%磷酸盐缓冲液(v/v/v=170∶10∶10),流速为1 mL/min,检测波长588 nm.结果表明:线性范围为0.04~0.20μg,金丝桃素的含量是0.21%(相当于每毫升注射液含金丝桃素0.009 3 mg),RSD为1.07%.HPLC法灵敏、准确、重现性好,可用于金丝桃素注射液含量的测定.
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| 关 键 词： | 金丝桃素注射液 高效液相色谱法 含量测定 |
| 文章编号： | 1003-4315(2008)02-0031-03 |
| 修稿时间： | 2007年5月11日 |
Preparation and content determination of hypericin injection |
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| XIE Deng-long,LIANG Jian-ping,HU Yong-hao,SHANG Ruo-feng,HUA Lan-ying,WANG Xue-hong.Preparation and content determination of hypericin injection[J].Journal of Gansu Agricultural University,2008,43(2):31-33. |
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| Authors: | XIE Deng-long LIANG Jian-ping HU Yong-hao SHANG Ruo-feng HUA Lan-ying WANG Xue-hong |
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| Abstract: | HPLC method was established for the determination of hypericin injection.The Chromsphere C18(4.6×150 mm,5 μm)column was used to determine the content of hypericin,and the mobile phase was methanol-acetonitrile-1 %phosphate buffer(170∶10∶10),the flow rate of 1ml/min and detection wavelength at 588 nm.The results showed that the calibration curve was linear within the quality range of 0.04～0.20 μg and hypericin content was 0.21 %(equivalent to 1 mL injection containing 0.009 3 mg hypericin),RSD was 1.07 %.It is suggest that HPLC could be used for ananlysis of compound hypericin injection with sensitive,accurate and highly reproductive. |
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| Keywords: | hypericin injection HPLC determination of content |
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