丽格海棠组织培养外植体再生体系建立关键技术的研究

以丽格海棠的叶、茎、花茎、叶柄等营养器官作为外植体进行组织培养,着重研究丽格海棠的最佳外植体,最佳培养基配方及接种模式的关键技术。研究表明初代培养基本培养基为MS,激素配比为6-BA0 5mg/L+NAA0 1mg/L,培养30d可获得较多的不定芽。外植体分化能力的研究表明丽格海棠的不同类型外植体能力大小为:叶>茎>花茎>叶柄;从叶片的不同部位来看,叶中>叶侧>叶边缘;从不同叶龄来看,中等成熟叶>幼叶>完全成熟叶>芽叶。叶片的接种模式:反放比正放好。

Study on the Technique System of Propagatio in vitro in Rieger Begonia

Leaves, sections of stems, flower stems, leafstalks of Rieger Begonia were used as explants in study of organ culture to establish a rapid technique system of propagation in vitro, and to find out the best explants, medium culture procedure. The results are as follows: Iniation medium: MS+6-BA 05?mg/L+NAA 01?mg/L adventitious buds can be got after 30 days culture. Effects of different explants on shoot regeneration: leaf＞stem＞flower stem＞leafstalk. Effects of different parts leaves on shoot regeneration: middle leaf＞lateral of leaf＞edge of leaf. Effects of different age of the leaves on shoot regeneration: medium mature leaf＞immature leaf＞mature leaf＞bud leaf. Effect of leaf inoculation model on shoot regeneration: while the leaves are placed on regeneration media with the abaxial surface in contact with the media, more adventitious shoots are obtaind from the leaves; While the leaves are placed on regeneration media with the adaxial surface in contact with the media, less adventitious shoots are obtaind from the leaves.