完整顶体精子注入卵细胞后对胚胎发育的影响

分别注射1、3、5个顶体完整的人精子于金黄地鼠卵中,对照组同时注射数量相等的无顶体精子,观察卵活化、受精及后期发育情况.结果发现随着注入精子数量的增加,卵发育的异常率显著增加,而注射无顶体精子组,无论注射精子数量为多少,均无异常发生.此外,注射多个精子于金黄地鼠卵中时,多原核(PN)形成率顶体完整组明显低于无顶体组(P...

A Study of Spermatozoa with Acrosome on Embryo Development

In this study,1,3 or 5 human spermatozoa with acrosomes injected into a hamster oocyte in experimental group,simultaneously,the same number spermatozoa without their acrosomes injected into control group hamster oocyte,oocytes activation,fertilization and post-development were observed.The results showed that the abnormal rate of oocytes development were significantly increased with the increasing of the number of spermatozoa injection.However,no matter how many number of spermatozoa injection,there were no abnormal oocytes in control group.In addition,when more human spermatozoa were injected into individual hamster oocytes,the multi-pronuclear formation rate of acrosomal integrity group were significantly lower than that of control group(P<0.01).Experimental results showed that spermatozoa acrosomal enzymes may have some influence on the eggs and embryonic post-development.The spermatozoa of removal acrosomes were more preferable and effective before ICSI.