| 5份金荞麦核糖体基因内转录间隔区(ITS)序列分析 |
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| 引用本文： | 赵海霞,白悦辰,陈惠,吴琦.5份金荞麦核糖体基因内转录间隔区(ITS)序列分析[J].四川农业大学学报,2011,29(3):299-303. |
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| 作者姓名： | 赵海霞 白悦辰 陈惠 吴琦 |
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| 作者单位： | 四川农业大学生命科学与理学院,四川雅安,625014 |
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| 摘 要： | 以5份金荞麦为材料,对其核糖体基因内转录间隔区(ITS)序列进行PCR扩增,均获得长约700 bp左右的特异性产物.测序结果表明,金荞麦样品中ITS序列在638～797 bp之间.经软件Clustal X2.0和MEGA 4.0分析结果表明,当空位作为缺失处理时,ITS区全序列包括488个保守位点,248个变异位点,6...
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| 关 键 词： | 金荞麦 ITS PCR 序列分析 |
Sequence Analysis of Internal Transcribed Spacer of Ribosome Gene among Five Fagopyrum cymosum Samples |
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| ZHAO Hai-xia,BAI Yue-chen,CHEN Hui,WU Qi.Sequence Analysis of Internal Transcribed Spacer of Ribosome Gene among Five Fagopyrum cymosum Samples[J].Journal of Sichuan Agricultural University,2011,29(3):299-303. |
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| Authors: | ZHAO Hai-xia BAI Yue-chen CHEN Hui WU Qi |
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| Institution: | ZHAO Hai-xia,BAI Yue-chen,CHEN Hui,WU Qi(College of Biology and Science,Sichuan Agricultural University,Yaan 625014,Sichuan,China) |
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| Abstract: | The genetic relationship among different Fagopyrum cymosum was revealed by analyzing the internal transcribed spacer(ITS) sequences of five F.cymosum samples.The sequencing results showed that there were about 700 bp including the whole ITS1,5.8S and ITS2 regions.The further analysis indicated that ITS regions of five samples were 638-797 bp in length.With lack of gap as treatment,the whole ITS sequence consisted of 488 conserved sites,248 variable sites,67 information sites and 175 singleton sites.The sequ... |
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| Keywords: | Fagopyrum cymosum ITS sequences PCR sequence analysis |
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