利用卡那霉素间接鉴定转基因抗虫棉的研究

研究了利用卡那霉素间接鉴定转基因抗虫棉的方法。结果表明,在棉花苗期使用8000mg/kg卡那霉素溶液处理子叶,或者在蕾期使用6000mg/kg卡那霉素溶液处理棉株顶部第2片展开叶,7～9d后非转基因棉株的叶片点液处出现黄色斑块,而转基因抗虫棉的叶片仍为正常绿色,从而简便有效地鉴别出转基因抗虫棉。经网室接虫测试和室内饲养生物学测试方法验证,该方法结果准确。

Study on the Identification of Transgenic Cotton by Using Kanamycin as an Indirect Marker

The identification method of transgenic cotton by using kanamycin as an indirect marker is presented. Dropping with 8000?mg/kg kanamycin solution on cotyledon in seedling stage, or with 6000?mg/kg kanamycin solution on the second expand leaves in bud stage, 7～9 days later, all of the leaves of non transgenic cotton plants show apparent yellow patches whereas the leaves of the transgenic cotton remain green without any symptoms. By using this method, the transgenic cotton plant can be identified effectively and simply, and the results are in agreement with results obtained by both the cage inoculating test and the insect resistance bioassay test.