铁皮石斛以芽繁芽离体培养技术体系的建立

【目的】建立铁皮石斛以芽繁芽离体培养技术体系，为广西铁皮石斛良种选育及种苗繁殖提供技术支持。【方法】以广西桂平种、容县种和西林种3种野生铁皮石斛后代材料茎段为外植体，进行丛生芽诱导、增殖和壮苗生根培养，探讨不同消毒时间和不同激素配比对丛生芽诱导培养等过程的影响。【结果】3种材料外植体的最佳消毒时间为10min，污染率仅10．0％-20．0％；桂平种丛生芽最佳诱导培养基为MS＋2．0mg／L6-BA＋0．2mg／LNAA＋10％椰子汁，丛生芽诱导率7．8％；容县种和西林种丛生芽诱导最佳培养基为MS＋1．5mg／L6-BA＋0．2mg／LNAA＋10％椰子汁，丛生芽诱导率分别为23．1％和30．8％。3种材料丛生芽继代增殖最佳培养基为MS＋0．5mg／L6-BA＋0．2mg／LNAA＋10％椰子汁，平均增殖倍数为3～5倍；壮苗生根最佳培养基为1／2MS＋0．5mg／LNAA＋0．2mg／LIBA＋10％香蕉泥，生根率在95．0％以上；以松树皮为移栽基质，移栽成活率在90．0％以上。【结论】以桂平种、容县种和西林种3种野生铁皮石斛后代材料茎段为外植体均可建立以芽繁芽离体培养技术体系；在诱导培养基和继代培养基中增加继代次数，可提高铁皮石斛的诱导率和增殖倍数。

In vitro culture technique of reproducing buds from buds of Dendrobium officinale Kimura et Migo

【Objective 】In vitro culture technique of reproducing buds from buds of Dendrobium officinale was established to provide technical support for selecting good varieties and propagating germchit of Dendrobium officinale in Guangxi. 【Method 】The effects of different sterilize time and different ratio of hormones on the inducing culture process of multiple shoots were researched by inducing, multiplying, strengthening and seeding the explants which were stems of wild Dendrobium officinale offspring materials form Guiping, Rongxian and Xilin. 【Result 】The best sterilize time of three materials was 10 min,and the contamination rate was 10.0%-20.0%. The best inducing medium of multiple shoots of Guiping ＇s material was MS ＋2.0 mg/L 6-BA ＋0.2 mg/L NAA ＋10 % coconut milk,in which inductivity was7.8%. The best inducing medium of multiple shoots of Rongxian and Xilin materials was MS ＋1.5 mg/L 6-BA ＋0.2mg/L NAA ＋10% coconut milk,in which inductivity was 23.1% and 30.8%. The best successive transfer culture medium of all these 3 materials was MS ＋6-BA 0.5 mg/L ＋NAA 0.2 mg/L ＋10 % coconut milk,in which average proliferation ratio was 3-5 times. The best rooting medium was 1/2MS ＋0.5 mg/L NAA ＋0.2 mg/L IBA ＋10 % banana puree,in which rooting percentage was more then 95.0%. The medium of transplanting was pine bark, in which surviving rate of transplant reached 90. 0 %. 【 Conclusion 】 All of these three stem explants from wild Dendrobium officinale offspring materials form Guiping, Rongxian, Xilin can be used to build an in vitro culture system of reproducing buds from buds. It can increase the inductivity and proliferation rate when increasing the transfer times on inducing medium and successive transfer culture medium.