HPLC法同时测定鼠尾草酸、鼠尾草酚和迷迭香酸的含量

为了探讨鼠尾草酸、鼠尾草酚和迷迭香酸同时测定的优化流程，对HPLC测定方法中的检测波长、精密度、稳定性、回收率等条件进行了筛选与优化。结果表明：迷迭香酸、鼠尾草酚、鼠尾草酸在5~100 mg·L-1的浓度范围内具有良好的线性，相关系数达到0.999以上，回收率分别为86.4%、85.6%和88.8%。针叶迷迭香(Rosmarinus officinalis "Pine")叶片中鼠尾草酸（25 585 mg·kg^-1）和鼠尾草酚（11 823 mg·kg^-1）含量最高，大叶迷迭香叶片中迷迭香酸含量最高（7 572.67 mg·kg^-1）。小叶迷迭香(Rosmarinus officinalis L.)茎中鼠尾草酸含量（1 570.00 mg·kg^-1）、鼠尾草酚含量（1 603.33 mg·kg^-1）和迷迭香酸含量（1 304.67 mg·kg^-1）在4个迷迭香品种中最高。HPLC测定方法稳定、可靠且高效，可为迷迭香非挥发性成分的开发与利用提供理论依据。

Contents of Carnosic Acid,Carnosol and Rosmarinic Acid Determined Simultaneously by HPLC

In order to optimize process of simultaneous determination of carnosic acid, carnosol and rosmarinic acid, the detection wavelength, precision, stability, repeatability and recovery rate of HPLC method were screened and optimized. The recovery rates of rosmarinic acid, carnosol and carnosic acid were 86.4%, 85.6% and 88.8%, respectively, with the correlation coefficient above 0.999 in the concentration range of 5~100 mg·L^-1. After testing it showed that the contents of carnosic acid (25 585 mg·kg^-1) and carnosol (11 823 mg·kg^-1) in the leaf of Rosmarinus officinalis "Pine"were the highest. The content of rosmarinic acid in the leaf of Rosmarinus officinalis "Rex" was the highest (7 572.67 mg·kg^-1). The highest contents of carnosic acid (1 570.00 mg·kg^-1), carnosol (1 603.33 mg·kg^-1) and rosmarinic acid (1 304.67 mg·kg^-1) were determinated in the stems of Rosmarinus officinalis L. among the four varieties. HPLC method was stable, reliable and efficient after optimization, which provided a basis for the development and utilization of non-volatile components of rosemary.