| 菟丝子RAPD反应体系的建立和优化 |
| |
| 引用本文： | 郭琼霞,黄可辉,庄蓉,黄振,虞赟,沈建国.菟丝子RAPD反应体系的建立和优化[J].江西农业学报,2008,20(5):21-24. |
| |
| 作者姓名： | 郭琼霞 黄可辉 庄蓉 黄振 虞赟 沈建国 |
| |
| 作者单位： | 1. 福建省出入境检验检疫局,福建,福州,350003
2. 福建农林大学,福建,福州,350002 |
| |
| 基金项目： | 福建科技重大专项资助项目
,
国家重大科技支撑计划 |
| |
| 摘 要： | 以南方菟丝子基因组DNA为试材,采用单因素递进筛选方法对菟丝子的RAPD反应体系进行了优化。结果表明,在25μL总反应体积中,最佳浓度配比为：模板DNA 20 ng,MgC l23.0 mmoL/L,引物10μmoL/L,dNTP 400μmoL/L,TaqDNA聚合酶1个单位（U）,10×反应缓冲液2.5μL。扩增程序为：94℃变性3 m in,再进入循环;94℃变性1 m in,40℃退火1m in,72℃延伸1 m in,共40个循环;72℃最终延伸10 m in,于4℃保存。
|
| 关 键 词： | 菟丝子 RAPD 反应体系 优化 |
| 文章编号： | 1001-8581(2008)05-0021-04 |
| 修稿时间： | 2008年1月17日 |
Establishment and Optimization of RAPD Reaction System of Cuscuta |
| |
| GUO Qiong-xia,HUANG Ke-hui,ZHUANG Rong,HUANG Zhen,YU Yun,SHEN Jian-guo.Establishment and Optimization of RAPD Reaction System of Cuscuta[J].Acta Agriculturae Jiangxi,2008,20(5):21-24. |
| |
| Authors: | GUO Qiong-xia HUANG Ke-hui ZHUANG Rong HUANG Zhen YU Yun SHEN Jian-guo |
| |
| Institution: | GUO Qiong - xia , HUANG Ke - hui ,ZHUANG Rong, HUANG Zhen,YU Yun ,SHEN Jian - gao (1. Fujian Entry- exit Inspection and Quarantine Bureau, Fuzhou 350003 ,China; 2. Fujian Agricultural and Forestry University, Fuzhou 350002, China) |
| |
| Abstract: | The genomic DNA of Cuscuta australis was extracted and was used to optimize the RAPD reaction system of Cuscuta.The results indicated that: the best reaction system was that 25 μL total reaction volume of RAPD-PCR contained 20 ng template DNA,3.0 mmol/L MgCl2,10 μmoL/L primer,400 μmoL/L dNTP,1 unit Taq DNA polymerase,10 times buffer 2.5 μL.The thermal cycle condition was: firstly denaturing at 94 ℃ for 3 minutes;then 40 cycles which involved denaturing at 94 ℃ for 1 minute,annealing at 40 ℃ for 1 minute,and elongation at 72 ℃ for 1 minute;finally elongation at 72 ℃ for 10 minutes,and then was kept at 4 ℃. |
| |
| Keywords: | Cuscuta RAPD Reaction system Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
