超声波辅助诱导春石斛原球茎途径再生体系的建立

春石斛是目前国际流行的高档盆花,研究春石斛品系材料No.H118和No.33原球茎再生体系的建立.首先通过原植株的茎尖或腋芽外植体诱导得到无菌苗;将无菌苗茎基无叶芽茎段,经40 kHz超声波预处理10 min,接于1/2 MS+6-BA 0.2 mg/L固体培养基可诱导得到原球茎,系列筛选试验表明,采用液体MS+6-BA 0.2 mg/L两供试材料均得到最高的原球茎增殖率,分别为4.57倍/月和4.31倍/月,添加150 mL/L的椰汁诱导效率显著高于添加蛋白胨和香蕉泥;采用1/2 MS+6-BA 1.0 mg/L+NAb.0.2 mg/L固体培养基,芽增殖率最高,不同的春石斛品种或材料原球茎诱导成功率存在显著性差异.

Establishment of Regeneration System of Spring Dendrobium Strain Induced with Ultrasonic Wave

Spring Dendrobium is a top-grade pot flower blooming in spring,which is popular in international flower market.In this paper,the regeneration system in vitro of Spring Dendrobium No.H118 and No.33 by PLBs was studied.To begin with,the plantlet in vitro was obmined from explants,then,PLBs were induced by basal stem without bud,which were pretreated with 40 kHz Ultrosorric Wave for 10 mines,then were respectively cultured in 1/2 MS+6-BA 0.2 mg/L solid culture medium and MS+6-BA 0.2 mg/L liquid medium for PLBs proliferation.Lastly proliferation rate of PLBs of Spring Dendrobium No.H118 and 33 Was obtained as 4.57 and 4.31 times per month in liquid medium.Culture by 1/2 MS+6-BA 1.0mg/L+NAA 0.2 mg/L solid medium for buds had great propagation rate.There were significant differences in ifiduction rates of PLBs among different materials of spring Dandrobium.