| 利用RAPD技术对桃种内种质的分析 |
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| 引用本文： | 袁政,罗来水,肖德兴,张大兵.利用RAPD技术对桃种内种质的分析[J].江西农业大学学报,2002,24(2):172-175. |
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| 作者姓名： | 袁政 罗来水 肖德兴 张大兵 |
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| 作者单位： | 1. 江西农业大学,农学院,江西,南昌,330045
2. 上海市农业科学院农业生物技术中心,上海市农业遗传育种重点实验室,上海,201106 |
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| 基金项目： | 国家自然科学基金资助项目 (396 6 0 0 4 8) |
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| 摘 要： | 利用随机扩增多态性DNA(RAPD)技术对毛桃 2 0个品种进行基因组DNA多态性分析 ,利用筛选出的14个 10碱基随机引物共扩增出 181条带 ,其中 14 3条具有自身特征性标记 ,利用这些DNA片段 ,按UPGMA法进行聚类分析 ,并建立树状图。在遗传距离 0 .70 2以上 ,将供试的 2 0个桃品种分为 5类 ,从分子生物学角度为桃种质的保存和育种提供理论依据
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| 关 键 词： | RAPD 桃 亲缘关系 |
| 文章编号： | 1000-2286(2002)02-0172-04 |
| 修稿时间： | 2001年11月20 |
A Study on the Genetic Relationship of Peach Species Using RAPD Markers |
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| YUAN Zheng ,LUO Lai-shui ,XIAO De-xin ,ZHANG Da-bing.A Study on the Genetic Relationship of Peach Species Using RAPD Markers[J].Acta Agriculturae Universitis Jiangxiensis,2002,24(2):172-175. |
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| Authors: | YUAN Zheng LUO Lai-shui XIAO De-xin ZHANG Da-bing |
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| Institution: | YUAN Zheng 1,LUO Lai-shui 1,XIAO De-xin 1,ZHANG Da-bing 2 |
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| Abstract: | Random amplified polymorphics DNA (RAPD) technique was used to analyze the genetic relationship of 20 species of peach (Prunus persica L.) and its relatives. Fourteen decanucleotide arbitrary primers were selected and 181 DNA fragments were generated, 143 of which were polymorphic. These fragments were used in the analysis of the relationship of the 20 species. A tree diagram was constructed by using UPGMA methods based on their genetic distance. Twenty varieties are divided into five types from the genetic distance 0.702. The studies provide theoretical basis for variety-keeping and breeding from molecular biology basis. |
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| Keywords: | RAPD peach relationship |
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