| 阿特拉津对黑土酶活及其微生物多样性影响研究 |
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| 引用本文： | 张颖,曹博,姜昭,张修远,王子懿,李瑞璇,张晋瑄.阿特拉津对黑土酶活及其微生物多样性影响研究[J].东北农业大学学报,2017,48(3). |
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| 作者姓名： | 张颖 曹博 姜昭 张修远 王子懿 李瑞璇 张晋瑄 |
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| 作者单位： | 东北农业大学资源与环境学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省应用技术研究与开发计划项目，农业部农业科研杰出人才及其创新团队，哈尔滨市应用技术研究与开发项目 |
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| 摘 要： | 以黑土为研究对象,采用室内培养方法,研究阿特拉津对黑土脲酶、转化酶、多酚氧化酶影响,采用RAPD技术分析土壤微生物群落。结果表明,低浓度(25 mg·kg-1)阿特拉津对黑土脲酶活性有促进作用,中、高浓度(50~125 mg·kg~(-1))阿特拉津对黑土脲酶活性具有抑制作用且浓度越高抑制越明显;阿特拉津抑制土壤转化酶活性,培养结束时(35 d),抑制作用仍然存在;阿特拉津对多酚氧化酶活性表现为抑制-促进-恢复规律;阿特拉津输入改变土壤微生物群落结构,浓度越高变化越明显。
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| 关 键 词： | 黑土 阿特拉津 微生物群落多样性 土壤酶 RAPD |
Effect of atrazine on soil enzyme activities and microbial community structure in black soil |
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| ZHANG Ying,CAO Bo,JIANG Zhao,ZHANG Xiuyuan,WANG Ziyi,LI Ruixuan,ZHANG Jinxuan.Effect of atrazine on soil enzyme activities and microbial community structure in black soil[J].Journal of Northeast Agricultural University,2017,48(3). |
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| Authors: | ZHANG Ying CAO Bo JIANG Zhao ZHANG Xiuyuan WANG Ziyi LI Ruixuan ZHANG Jinxuan |
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| Abstract: | The aim of this work was to evaluate the effect of atrazine on the activity of black soil urease,invertase,polyphenol oxidase by simulated experiments,while soil microbial communities was detected by using RAPD technology.The results showed that low concentration of atrazine (25 mg· kg-1) had simulative effect on the activity of soil urease.Other concentrations of atrazine (50-125 mg· kg-1) showed inhibitory effect on the activity of soil urease and the inhibition effect was enhanced with the concentration increasing.Atrazine restrained the activity of soil invertase throughout the culture period.However polyphenol oxidase in black soil showed a pattern of inhibition-activation-recovery.In addition,the structure of soil microbial community was changed by atrazine and the change was enhanced with the increase in atrazine concentration. |
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| Keywords: | black soil atrazine microbial community diversity soil enzymes RAPD |
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