| 鸡免疫球蛋白 GFc(IgGFc)重链的分离纯化及其抗血清制备——Ⅰ 兔抗鸡 IgGFc 重链抗血清制备 |
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| 引用本文： | 李庆章,刘忠贵.鸡免疫球蛋白 GFc(IgGFc)重链的分离纯化及其抗血清制备——Ⅰ 兔抗鸡 IgGFc 重链抗血清制备[J].东北农业大学学报,1990(2). |
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| 作者姓名： | 李庆章 刘忠贵 |
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| 作者单位： | 东北农学院兽医病理教研室，东北农学院兽医病理教研室 |
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| 摘 要： | 经硫酸铵盐析沉淀、柱色谱分离纯化得到鸡血清 IgG。然后用木瓜蛋白酶水解IgG,再经 DEAE-纤维素柱色谱纯化制得 IgGFc 重链。继用 IgGFc 重链免疫家兔制备兔抗鸡 IgGFc 重链抗血清。
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| 关 键 词： | 鸡 IgG TgGFc 重链 抗血清 |
ISOLATION AND PURIFICATION OF CHICKEN IMMUNOGLOBULIN GFc(IgGFc)HEAVY CHAIN AND PREPARATION OF ITS ANTISERA——1.Preparation of Rabbit Anti-chicken IgGFc Heavy Chain Antisera |
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| Li Qingzhang Liu Zhonggui.ISOLATION AND PURIFICATION OF CHICKEN IMMUNOGLOBULIN GFc(IgGFc)HEAVY CHAIN AND PREPARATION OF ITS ANTISERA——1.Preparation of Rabbit Anti-chicken IgGFc Heavy Chain Antisera[J].Journal of Northeast Agricultural University,1990(2). |
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| Authors: | Li Qingzhang Liu Zhonggui |
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| Abstract: | Immunoglobulin G(IgG)in chicken serum was separated and purified by salting-out of ammonium sulfate and column chromatography.Papainwas used to digest chicken IgG. IgGFc heavy chain was obtained on DEAE-cellulose column chromatography.The prepara- tion of rabbit anti-chicken IgGFc heavy chain antisera was completed after rabbits were im- muned with IgGFc heavy chain. |
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| Keywords: | chicken IgG IgGFc heavy chain antiscra |
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