KDR等6个基因多态性与鸡生长和体组成性状的相关研究

以东北农业大学高、低脂肉鸡双向选择品系第14世代群体(NEAUHLFG14)和AA肉鸡商业随机群体(AA群体)为试验材料,利用PCR-RFLP对鸡腹脂性状的全基因组关联分析(GWAS)鉴定出6个多态位点进行单核苷酸多态性(SNPs)检测,分析这些位点的多态性与鸡生长和体组成性状的关系。结果表明,在NEAUHLFG14中KDR基因的rs14483313位点多态性对腹脂重(AFW)和腹脂率(AFP)有显著影响(P<0.05),TUSC3基因的rs13548811位点对鸡后期体重有影响,EXOC1基因的GGaluGA263078位点在两个群体中都对7周龄体重(BW7)有显著影响(P<0.05)。TUSC3、PPAT、EXOC1和KIAA1211基因的突变位点对NEAUHLFG14极低密度脂蛋白(VLDL)浓度有显著影响(P<0.05),结果显示,6个基因可能是影响鸡腹脂性状的重要基因,但多态位点是否为功能性位点仍需进一步验证。

Association of six genes including KDR polymorphisms with growth and body composition traits in chicken

Six SNPs identified by GWAS of chicken's abdominal fat trait were detected by PCR-RFLP in fourteenth generation population of the chicken divergently selected for abdominal fat content of Northeast Agricultural University(NEAUHLFG14) and Arbor Acres(AA population) commercial random population,and the association between the SNPs and growth,and body composition traits were performed.The results showed that rs14483313 locating in the KDR gene significantly affected the AFW and AFP(P<0.05),and rs13548811 residing in the TUSC3 gene affected the later body weight traits in NEAUHLFG14.GGaluGA263078 in the EXOC1 gene was significantly associated with BW7 in both the NEAUHLFG14 and AA population(P<0.05).In addition,the loci residing in TUSC3,PPAT,EXOC1 and KIAA1211 were significantly associated with VLDL in the NEAUHLFG14(P<0.05).Based upon the resuts in the present study,it is suggested that the six genes studied could influnce abdominal fat traits in chickens,but whether these loci are causal mutations or not needs to be confirmed further.