| 8种随机引物扩增鳜鱼病毒核酸的初步研究 |
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| 引用本文： | 李新辉,吴淑勤,李凯彬,白俊杰,潘厚军,罗建仁,简清.8种随机引物扩增鳜鱼病毒核酸的初步研究[J].大连水产学院学报,2001,16(1):57-60. |
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| 作者姓名： | 李新辉 吴淑勤 李凯彬 白俊杰 潘厚军 罗建仁 简清 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所, |
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| 基金项目： | 农业部“九五”重点科研项目 !(95-A -96-0 3 -0 1 )，广东省自然科学基金资助项目 !(980 90 7) |
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| 摘 要： | 从感染鳜鱼病毒（Siniperca chuatsi virus)的鳜鱼中分离病毒，提纯核酸作模板，用8种带酶切位点的引物，进行病毒酸随机扩增，探讨了在多聚酶链反应（RCR）过程中，引物长度，Mg^2 浓度和生温度对获得扩增带谱的影响。
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| 关 键 词： | 鳜鱼病毒 核酸 PCR扩增 RAPD技术 鳜鱼 流行病 |
| 文章编号： | 1000-9957(2001)01-0057-04 |
Analysis of nucleic acid on Siniperca chuatsi virus using eight kinds of random primer |
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| LI Xin-Hui,WU Shu-qin,LI Kai-Bin,BAI Jun-jie,PAN Hou-jun,LUO Jian-ren,JIAN Qing.Analysis of nucleic acid on Siniperca chuatsi virus using eight kinds of random primer[J].Journal of Dalian Fisheries University,2001,16(1):57-60. |
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| Authors: | LI Xin-Hui WU Shu-qin LI Kai-Bin BAI Jun-jie PAN Hou-jun LUO Jian-ren JIAN Qing |
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| Abstract: | Virual nuclei acid was isolated from the mandarin fish infected \%Siniperea chuatsi virus\% (SCV). The nucleic acid was used as PCR templet after purified from low melting agros. Eight random primers containing enzyme position were used to amplify SCV genome in this experiment. Influence of primer length, concentrotion of Mg 2+ , temperature on annealing was studied during PCR. |
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| Keywords: | Siniperea chuastsi virus\% nucleic acid RAPD |
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