氧化鱼油对鲤肝胰脏抗氧化机能

在半纯化饲料中添加质量分数为３％的新鲜鱼油（过氧化物值ＰＯＶ为０．６４ｍｍｏｌ／ｋｇ）和ＰＯＶ分别为２９．６４、５９．４０和９４．６９ｍｍｏｌ／ｋｇ（以Ｏ２计）的氧化鱼油，投喂１００ｇ左右２龄鲤（Ｃｙｐｒｉｎｕｓ ｃａｒｐｉｏ）鱼种１５周，结果表明：鲤摄食氧化鱼油后，肝胰脏维生素Ｅ含量下降（Ｐ〈０．０５），抗氧化酶超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性减弱（Ｐ〉０．０５），微粒体氧化稳定性降低（Ｐ〈０．０５），鱼渔氧化程度升高，上述变化加强；肝胰脏脂肪含量增加（Ｐ〉０．０５）；肾脏和脾脏增生（Ｐ〉０．０５）；肝胰脏肝细胞纤维化、线粒体嵴降解和融合。上述系列变化表明氧化钱油可以破坏鲤肝胰脏抗氧化机能及其正常的组织结构。

Effects of oxidized fish oil on the anti-oxidative function and histological structure of carp hepatopancreas

Juvenile carp (\%Cyprinus carpio\%) weighing 100 g were fed a semi purified diet supplemented with 3% of fresh fish oil (Peroxide value, POV, 0.64 mmol/kg) or oxidized fish oil at POV levels of 29.64, 59.40, or 94.69 mmol/kg for 15 weeks. Oxidized fish oil decreased hepatopancreas vitamin E contents (\%P\%<0.05) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) (\%P\%>0.05) and microsomal oxidative stabilities (\%P\%<0.05). These trends were even worse when oxidized degrees of fish oil increased. With the oxidized fish oil used in the diet, fat content in hepatopancreas was elevated (\%P\%>0.05) and kidney somatic indices (KSI) and spleen somatic indices (SSI) were enhanced (\%P\%>0.05). In hepatopancreas of carp fed diet with oxidized fish oil, pronounced histological abnormalities were noted including liver cell fibrillation and degenerated and melted cristae of mitochondria. These changes reveal that oxidized fish oil destroyed anti oxidative function and normal histological structure of hepatopancreas.