| 养殖大口黑鲈的遗传多样性分析 |
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| 引用本文： | 梁素娴,白俊杰,叶星,劳海华,简清.养殖大口黑鲈的遗传多样性分析[J].大连水产学院学报,2007,22(4):260-263. |
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| 作者姓名： | 梁素娴 白俊杰 叶星 劳海华 简清 |
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| 作者单位： | 1. 中国水产科学研究院,珠江水产研究所,农业部热带亚热带鱼类选育与养殖重点开放实验室,广州,510380;大连水产学院,生命科学与技术学院,辽宁,大连,116023
2. 中国水产科学研究院,珠江水产研究所,农业部热带亚热带鱼类选育与养殖重点开放实验室,广州,510380 |
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| 基金项目： | 科技部科技基础条件平台建设计划 |
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| 摘 要： | 用RAPD技术分析了广东养殖大口黑鲈Micropterus salmoides 3个群体基因组DNA的多态性.结果表明:用12条随机引物对3个群体共88尾大口黑鲈的基因组DNA进行扩增,共获得了106个位点,平均每条随机引物扩增出8.8个位点;广州群体、顺德大良群体和顺德南水群体的多态位点比例分别为32.08%、33.02%和40.57%;Nei遗传多样性指数分别为0.1151、0.1176和0.1627;Shannon's遗传多样性指数分别为0.1708、0.1742和0.2378.这表明目前广东地区养殖大口黑鲈种质资源还具有一定的遗传多样性,但养殖群体的遗传多样性在降低,且具有不同程度的种质退化.
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| 关 键 词： | 大口黑鲈 遗传多样性 RAPD |
| 文章编号： | 1000-9957（2007）04-0260-04 |
| 修稿时间： | 2006-09-01 |
RAPD analysis of genetic diversity for cultured largemouth bass Micropterus salmoides |
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| LIANG Su-xian,BAI Jun-jie,YE Xing,LAO Hai-hua,JIAN Qing.RAPD analysis of genetic diversity for cultured largemouth bass Micropterus salmoides[J].Journal of Dalian Fisheries University,2007,22(4):260-263. |
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| Authors: | LIANG Su-xian BAI Jun-jie YE Xing LAO Hai-hua JIAN Qing |
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| Abstract: | Random amplified polymorphic DNA(RAPD) method was used to detect the genetic diversity of cultured largemouth bass Micropterus salmoides obtained from Guangdong province.Using 12 random primers,106 RAPD sites were detected,with the average loci of 8.8 in each primer.The Guangzhou,Shunde and Nanshui populations had mean polymorphic loci percentage of 32.08%,33.02% and 40.57%;Nei's gene diversity of 0.1151,0.1176 and 0.1627 and the Shannon's diversity of 0.1708,0.1742 and 0.2378,respectively.These indexes indicate that the cultured largemouth bass from Guangdong province has not decrease in genetic diversity at present, but in some areas has decrease. |
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| Keywords: | Micropterus salmoides genetic diversity RAPD |
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