| HPLC法测定菌株S15发酵液中Zygosporin D的含量 |
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| 引用本文： | 伍陈浔利,黄玖利,王辉,戴好富,梅文莉.HPLC法测定菌株S15发酵液中Zygosporin D的含量[J].热带农业科学,2012,32(4):83-86,91. |
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| 作者姓名： | 伍陈浔利 黄玖利 王辉 戴好富 梅文莉 |
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| 作者单位： | 1. 海南大学环境与植物保护学院,海南海口,570288
2. 中国热带农业科学院热带生物技术研究所／农业部热带作物生物技术重点开放实验室,海南海口,571101 |
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| 基金项目： | 国家自然科学基金(No.30560018);国家重点基础研究发展计划(No.2009CB1259900);中央级公益性科研院所基本科研业务费专项资金项目(No.ITBBZD0841);海南大学“211工程”建设项目 |
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| 摘 要： | 用HPLC法测定菌株S 15发酵液中Zygosporin D(乙酰基细胞松弛素D)的含量。色谱条件为:Kromasil-C18柱(4.6 mm×150 mm,5 m),柱温25℃,流动相乙腈-水(体积比40:60),流速(1.0 mL/min),检测波长(216nm)。标准曲线回归方程:Y=0.409 03X+1.547 7,r=0.999 8,Zygosporin D回收率为100.5%。实验证明该方法稳定可靠,精密度高,重现性好,简单可行,适用于Zygosporin D的分析检测。
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| 关 键 词： | S15发酵液 去乙酰基细胞松弛素D HPLC |
Determination of Zygosporin D in S15 Fermentation Liquor by RP-HPLC |
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| WUCHEN Xunli,HUANG Jiuli,WANG Hui,DAI Haofu,MEI Wenli.Determination of Zygosporin D in S15 Fermentation Liquor by RP-HPLC[J].Chinese Journal of Tropical Agriculture,2012,32(4):83-86,91. |
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| Authors: | WUCHEN Xunli HUANG Jiuli WANG Hui DAI Haofu MEI Wenli |
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| Institution: | 1 College of Environment and Plant Protection,Hainan University,Haikou,Hainan 570288; 2 Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology/Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou,Hainan 571101) |
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| Abstract: | HPLC was used to determine the content of Zygosporin D in S15 fermentation liquor.The sample was analyzed on Kromasil-C18(4.6 mm×150 mm,5 m) at 25℃ with the mobile phase of Acetonitrile-water(40∶60),the flow rate of(1.0 mL/min),and the detection wave length of 216 nm.The calibration curve equation was Y=0.409 03X+1.4653(r=0.999 8).A recovery rate of 100.5% was achieved.The results proved that this method was suitable for detecting Zygosporin D with good stability,high precision,strong reproducibility,and simple operation. |
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| Keywords: | S15 fermentation liquor Zygosporin D RP-HPLC |
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