| HPLC紫外法同时检测生鲜乳中环丙沙星和恩诺沙星残留 |
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| 引用本文： | 陈海荣,吕世明,谭艾娟等.HPLC紫外法同时检测生鲜乳中环丙沙星和恩诺沙星残留[J].安徽农业科学,2014(6):1724-1725,1728. |
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| 作者姓名： | 陈海荣 吕世明 谭艾娟等 |
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| 作者单位： | [1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州省兽药监察所,贵州贵阳550003 |
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| 基金项目： | 贵州省科技厅农业公关项目（黔科合NZ字[2012]3011号）;贵阳市农委科学技术计划项目（筑科合（2009第12-1号）. |
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| 摘 要： | 目的]建立能同时检测环丙沙星和恩诺沙星残留的HPLC紫外检测法。方法]采用10%三氯乙酸为提取液,直接从生鲜乳中提取环丙沙星和恩诺沙星,并进行HPLC检测。结果]环丙沙星和恩诺沙星的最低检测限为5μg/L,在10~200μg/L浓度范围内呈良好的线性关系(r0.99),平均回收率为84%~112%,变异系数均小于5%。结论]该方法不仅除蛋白和脂肪效果好,而且回收率较为理想,可以很好满足确证方法的要求,且重现性好。
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| 关 键 词： | 恩诺沙星 盐酸环丙沙星 高效液相色谱法 生鲜乳 残留 |
Simultaneous Determination of Ciprofloxacin Hydrochloride and Enrofloxacin Residues in Raw Fresh Milk by HPLC-ultraviolet Method |
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| Institution: | CHEN Hai-rong,LV Shi-ming,TAN Ai-juan( 1.College of Animal Sciences, Guizhou University, Guiyang, Guizhou 550025; 2.College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025;) |
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| Abstract: | Objective] The research ained to establish HLPC-ultraviolet detection method for simultaneous determination of ciprofloxacin hydrochloride and enrofloxacin residues.Method] Taking 10％ trichloroacetic acid as extracting solution,ciprofloxacin hydroehloride and enrofloxacin were directly extracted from raw fresh milk to make HPLC detection.Resuh] The lowest detection limit of ciprofloxaein hydroehloride and enrofloxacin was 5 μg/L and there was a good linear correlation in the range of 10-200 μg/L（r ＞ 0.99）.The average recovery rate was 84％-112％ and the variation coefficient was less than 5％.Conclusion] The method had better removal effects of protein and fat and the recovery rate was ideal.The method could better meet the demands of the confirmatory methods and the repeatability was good. |
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| Keywords: | Enrofloxacin Ciprofloxacin hydroehloride HPLC Raw fresh milk Residue |
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