| 亚麻籽饼粕中开环异落叶松树脂酚二葡萄糖苷的提取工艺研究 |
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| 引用本文： | 陈志强,高岚,王昌禄.亚麻籽饼粕中开环异落叶松树脂酚二葡萄糖苷的提取工艺研究[J].安徽农业科学,2008,36(3):855-856. |
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| 作者姓名： | 陈志强 高岚 王昌禄 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津市食品营养与安全重点实验室,天津,300457;天津市尖峰天然产物研究开发有限公司,天津,300457
2. 天津科技大学食品工程与生物技术学院,天津市食品营养与安全重点实验室,天津,300457 |
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| 摘 要： | 建立了定性分析亚麻籽饼粕中开环异落叶松树脂酚二葡萄糖苷(SDG)的TLC方法,根据TLC结果,确定提取溶剂为0.1 mol KOH溶于1 L 70%乙醇,固液比为1∶8,提取2次。然后用大孔吸附树脂与TLC相结合的方法,探讨了亚麻籽中SDG的分离纯化,并根据HPLC分析结果确定了最佳的分离纯化工艺条件。即当亚麻籽饼粕KOH-乙醇-水提取液经中和浓缩后,用DM-21型大孔吸附树脂吸附,水洗去杂质,60%乙醇洗脱,浓缩干燥,经HPLC检测,亚麻籽饼粕含SDG为1.64%,粗提物产率达4.98%,SDG质量分数为32.93%。结果表明:KOH-乙醇-水提取与大孔吸附树脂相结合制备SDG提取物的方法与传统分离法相比,得率高、工艺操作简单且安全无毒。
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| 关 键 词： | 亚麻籽饼粕 亚麻木酚素 TLC HPLC |
| 文章编号： | 0517-6611（2008）03-00855-02 |
| 收稿时间： | 2007-09-24 |
| 修稿时间： | 2007年9月24日 |
Study on the Extraction Technique of Secoisolarieiresinol Diglucoside in the Flaxseed Cake |
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| CHEN Zhi-qiang et ai.Study on the Extraction Technique of Secoisolarieiresinol Diglucoside in the Flaxseed Cake[J].Journal of Anhui Agricultural Sciences,2008,36(3):855-856. |
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| Authors: | CHEN Zhi-qiang et ai |
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| Abstract: | The lignan secoisolariciresinol diglucoside(SDG) is a bioactive molecule effective on anticancer,antivirus and antioxidation.A simple and direct method for SDG qualitative analysis was established with TLC.Suitable macroporous adsorption resin(MAR DM-21) was chosen to separate SDG from extract solvent system(KOH-ethanol-water) of flaxseed cake.The identification of SDG and the determination of the purity of targeted fraction were carried out with HPLC.It was found that DM-21 showed an extract rate as 4.98% and the purified fraction contained 32.93%(W/W)SDG.Compared to the conventional extraction method,the yield of SDG was improved significantly and the extract time was greatly reduced.It was considered as a safe and suitable medium for scaled-up purification of SDG |
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| Keywords: | Flaxseed cake SDG TLC HPLC |
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